To explore the biological mechanism of Parkinson's disease（PD）of kidney essence deficiency syndrome from the perspective of glucose metabolism and the mechanism of intervention of Dihuang Yinzi（地黄饮子）in PD of kidney essence deficiency syndrome.

Fifty-five SPF grade male 12-week-old SAM-P8 mice were divided into model group，positive drug group，Dihuang Yinzi low，middle and high dose groups，with 11 mice in each group，and 11 SAM-R1 mice were as the normal group. The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine（MPTP）（30 mg/kg，once a day）into SAM-P8 mice for 7 days，and the intervention lasted for 4 weeks. The general status indicators of mice was observed，and the fasting blood glucose levels and behavioral indicators in mice were measured. HE staining was used to observe the structural changes of mouse kidney and testis. Western blot（WB），immunohistochemistry and polymerase chain reaction（PCR）were used to detect the protein contents of glucose transporter 1（GLUT1），glucose transporter 3（GLUT3），glucose transporter 4（GLUT4），and glycogen synthase kinase 3β（GSK-3β）in the substantia nigra and striatum of mouse brain tissue.

In the open field experiment，compared with the normal group，the model group showed a decrease in total exercise distance（P<0.05）and an increase in latency（P<0.05）. Compared with that of the model group，the latency of mice in the Dihuang Yinzi low and high dose groups decreased（P<0.05）. The total distance of mice in the Dihuang Yinzi middle dose group increased（P<0.05）. In the pole climbing experiment，compared with the normal group，the model group showed an increase in pole climbing time（P<0.05）. Compared with the model group，the Dihuang Yinzi low and high groups showed a decrease in time（P<0.05）. Compared with those before administration，the Dihuang Yinzi low，middle and high dose groups showed a decrease in time（P<0.05）. In the gait experiment，compared with the normal group，the model group showed significant differences in right anterior contact area，left anterior propulsion time，right posterior propulsion index，left posterior support phase and right anterior support phase（P<0.01）. Compared with that in the model group，there was a significant difference in the right posterior propulsion index（P<0.01）in the Dihuang Yinzi low dose group. There were significant differences in the contact area of the right forelimb，right posterior propulsion index，left posterior support phase，and right anterior support phase in the Dihuang Yinzi middle dose group（P<0.01）. There were significant differences in the contact area of the right forelimb，left posterior support phase and right anterior support phase in the Dihuang Yinzi high dose group（P<0.01）. HE renal staining test results showed compared with the blank group，the model group showed pathological changes such as glomerular epithelial cell proliferation，increased lipid droplets，renal tubular dilation，increased cell debris，increased red blood cells，increased lymphocytes，increased fibroblasts and increased fibroblasts. Compared with the model group，the Dihuang Yinzi high dose group showed a decrease in lipid droplets，red blood cells and fibroblasts. The HE testicular staining results showed that compared with the blank group，the model group showed pathological changes such as vacuolization of the spermatogenic epithelium and decreased sperm count. Compared with the model group，the Dihuang Yinzi high dose group showed reduced vacuolization of the spermatogenic epithelium. WB detection results showed compared with the normal group，the model group showed a significant decrease in GLUT1 protein expression in the substantia nigra（P<0.01），and a decrease in protein expressions of GLUT3 and GLUT4（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed a significant increase in protein expressions of GLUT1 and GLUT4 protein expression（P<0.01，P<0.05）and GSK-3βprotein expression decreased（P<0.05）. Compared with the normal group，the protein expressions of GLUT1 and GLUT3 in the striatum of the model group decreased significantly（P<0.01），and the expression of GLUT4 protein decreased（P<0.05）. The protein expression of GSK-3βincreased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed an increase in protein expression of GLUT1，GLUT3 and GLUT4（P<0.05，P<0.01），and GSK-3βprotein expression decreased（P<0.05）. Real time fluorescence quantitative PCR detection results showed compared with the normal group，the mRNA expressions of GLUT1，GLUT3 and GLUT4 in the substantia nigra of the model group mice were significantly reduced（P<0.01），and GSK-3βexpression increased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed increased expressions of GLUT1，GLUT3 and GLUT4（P<0.05），and GSK-3βexpression decreased（P<0.05）. Compared with the normal group，the mRNA expressions of GLUT1 and GLUT3 in the striatum of the model group were significantly lower（P<0.01），the expression of GLUT4 was lower（P<0.05）and the expression of GSK-3βincreased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed increased expressions of GLUT1，GLUT3 and GLUT4（P<0.05），and GSK-3βexpression decreased（P<0.05）. Immunohistochemical test results showed compared with the normal group，the expressions of GLUT1 and GLUT4 in the substantia nigra of the model group decreased（P<0.05），and GSK-3βexpression increased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group significantly increased the expression of GLUT1（P<0.01）. Compared with the normal group，the expressions of GLUT1 and GLUT4 in the striatum of the model group decreased significantly（P<0.01，P<0.05），respectively. Compared with the model group，the Dihuang Yinzi high dose group significantly increased the expressions of GLUT1，GLUT3 and GLUT4（P<0.01，P<0.05），and the expression of GSK-3βdecreased（P<0.05）.

1. Dihuang Yinzi can improve the motor speed，grip ability，limb coordination and exploration ability of PD mice with kidney essence deficiency syndrome.2. Dihuang Yinzi can reduce fasting blood glucose in mice with PD kidney essence deficiency syndrome，improve energy metabolism by correcting glucose metabolism.3. Dihuang Yinzi can increase the protein expressions of GLUT1 and GLUT4 in the substantia nigra of PD mice with kidney essence deficiency syndrome，and reduce the protein expression of GSK-3βin the substantia nigra of the brain，increase the expressions of GLUT1，GLUT3 and GLUT4 in the brain striatum，and reduce the expression of GSK-3β in the brain striatum. The protein expression in the Dihuang Yinzi middle and high dose groups was higher than that in the Dihuang Yinzi low dose group.4. Dihuang Yinzi has abnormal intake of GLUT1，GLUT3，GLUT4 and GSK-3βproteins. Dihuang Yinzi can treat PD of kidney essence deficiency syndrome by regulating the expressions of GLUT1，GLUT3，GLUT4 and GSK-3β proteins in the substantia nigra and striatum of PD mice.