Full
Outline
Autophagy is involved in the pathological changes of traumatic optic neuropathy（TON），and the regulation of autophagy mediated by the adenosine monophosphate activated kinase（AMPK）-mammalian target of rapamycin（mTOR）-ULK pathway is a potential therapeutic approach.AstragalosideⅣ（AS-Ⅳ）can regulate autophagy and plays a therapeutic role in a variety of diseases.This study aimed to observe the therapeutic effect of AS-Ⅳon TON and the role of AMPK-mTOR-ULK pathway-mediated autophagy in this process.
The rats were divided into seven groups：sham operation group，model group，AS-Ⅳlow，medium and high dose groups，rapamycin group，AMPK inhibitor group（AS-Ⅳ+compound C）.Correlation tests were performed after 7 days of the corresponding drug intervention.
After 7 days，the phosphorylation levels of AMPK，mTOR and ULK proteins in retinal tissue changed significantly after TON.AS-Ⅳwas found to increase LC3 mRNA and protein levels in TON model rats in the study.In addition，it increased the levels of p-AMPK and p-ULK and reduced p-mTOR level.Moreover，AS-Ⅳincreased the survival rate of retinal ganglion cells（RGCs）and improved the P2 peak latency time of flash-visual evoked potential（F-VEP）.The above effects were reversed by the AMPK inhibitor compound C.
AS-Ⅳcan increase the survival rate of RGCs and improve visual function after TON，which is related to the improvement of autophagy by regulating the AMPK-mTOR-ULK pathway.The AMPK-mTOR-ULK pathway may be a potential therapeutic approach for TON.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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