Full
Outline
Using Box-Behnken design response surfacemethodology to optimize the preparation process parameters of magnololwhey protein isolate nanoparticles（MAG-WPINPs），and to characterize their formulation and evaluate their antioxidant and antibacterialeffects.
MAG-WPI-NPswere prepared using a desolvation method，with encapsulation efficiency as the evaluation index. The preparation process parameters were optimized using a single factor combined with Box-Behnken design response surface methodology. The optimized MAG-WPI-NPs were characterized by their morphology，particle size，potential and other physicochemical properties. The free radical scavenging ability of 1，1-diphenyl-2-trinitrophenyl hydrazine（DPPH）and its ability to inhibit Escherichia coli were evaluated.
The optimal preparation process parameters included a volume ratio of12：1 for anti solvent/solvent，a feed ratio of12：1 for whey protein/magnolol，and a probe time of 1.5 minutes. The prepared MAG-WPI-NPs had a clear，transparent and slightly bluish appearance. Under transmission electron microscopy，the nanoparticles appear spherical with an average particle size of（227.33±0.02）nm. The potential was（-23.6±0.02）mV，the encapsulation efficiency was（98.57±0.46）%，the drug loading was（8.57±0.001）%，and the polydispersity index（PDI）was（0.1369±0.26）%. MAG-WPI-NPs had DPPH radical scavenging effects，Escherichia coli inhibitory effects，and were more potent than free drugs.
This study prepared a natural food grade self-assembled MAG-WPI-NPs. The preparation method was simple，safe and non-toxic，significantly improving the poorwater solubility and low bioavailability of magnolol and laying the foundation for the clinical application of magnolol.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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