Full
Outline
To observe the effects of Huangqi（Astragali Radix）on the expressions of key enzymes in the vitamin D system（CYP27B1，CYP24A1）and the levels of1，25（OH）2D3 and IL-22 in the skin lesions of amouse psoriasis likemodel induced by imiquimod.
Successfully modeled mice were random ly divided into amodel control group，a vitamin D group，a methotrexate group and Huangqi（Astragali Radix）low，medium and high dose groups. A normal control group was also established，and the continuous intervention was given for 3 weeks. The skin lesions were evaluated by psoriasis area and severity index（PASI）. Hematoxylin-eosin stain（HE）staining was used to observe the pathological changes of mouse skin lesions. Enzyme-linked immunosorbent assay（ELISA）detected the levels of serum 1，25（OH）2D3 and IL-22 in mice. Real TimeqPCR and immunohistochemistry methods were used to detect the mRNA and protein expressions of CYP27B1 and CYP24A1 in mouse skin lesions，respectively.
Compared with the normal control group，themodel group showed significant changes in skin lesions，a significant increase in PASI score（P<0.05），characteristic pathological changes in psoriasis，a significant decrease in serum 1，25（OH）2D3 levels（P<0.05），a significant increase in IL-22 levels（P<0.05），a significant down-regulation of CYP27B1 expression in skin lesions（P<0.05），and a significantup-regulation of CYP24A1 expression（P<0.05）. Compared with the model control group，the PASI scores of the Huangqi（Astragali Radix）medium and high dose groups，the vitamin D group and methotrexate group，were significantly reduced（P<0.05），and the tissue pathology improved. The levels of 1，25（OH）2D3 were significantly increased（P<0.05），and the levels of IL-22 were significantly reduced（P<0.05）. The expression of CYP27B1 in skin lesions was significantly up-regulated（P<0.05），and the expression of CYP24A1 was significantly down-regulated（P<0.05）.
Huangqi（Astragali Radix）can improveIL-22 levels in psoriasismice，which may be related to regulating the expression of key enzymes CYP27B1 and CYP24A1 in the vitamin D system.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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