Full
Outline
To analyze the effect of ursolic acid on the expressions of long chain non coding RNA（lncRNA）-LINC01089 and its targetgenes in papillary thyroid carcinoma（PTC）.
LINC01089 was a differentially expressed lncRNA that was previously screened and verified by transcriptome sequencing in the early stage. TPC-1 cells and normal human thyroid cells（Nthy-ori3-1）were routinely cultured in vitro and were divided into normal human thyroid cell group，control group and UA groups（10，20，40 μmol·L-1）. The survival rate of TPC-1 cells in each group was detected by CCK-8 assays. Scratch and Transwell experiments were conducted to investigate the effects of different concentrations of ursolic acid on the horizontal，vertical migration and invasion of TPC-1 cells. RT qPCRmethod was used to detect the relative expression level of LINC01089 in TPC-1 cells and the intervention effect of ursolic acid. RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of target genes Ras Homolog Family Member F（RHOF）and 4-Hydroxyphenylpyruvate Dioxygenase（HPD）as well as the intervention effectof ursolic acid.
Different concentrations of UA（10，20，40 and 80 μmol·L-1）could inhibit the proliferation of TPC-1 cells for different periods of time（12～48 hours）. When the concentration of ursolic acid was 10～40 μmol·L-1，the inhibitory effect of the cells gradually increased with increasing of concentration and prolongation of action time within 12 and 24 hours（P<0.05）. After treating TPC-1 cells with ursolic acid for 24 hours，the cell proliferation was slow，and as the concentration of ursolic acid（10，20 and 40 μmol·L-1）increased，morphological changed in cell apoptosis. Compared with the control group，the ursolic acid（10，20，40 μmol·L-1）groups inhibited the migration and invasion of TPC-1 cells in a significant concentration dependent manner（P<0.01）. Ursolic acid can up-regulate the expression of LINC01089，and down-regulate themRNA and protein expression levels of RHOF and HPD.
Ursolic acid has a certain inhibitory effect on thyroid papillary carcinoma cells，and its mechanism may be related with the LINC01089 and its target gene RHOF.
40
18
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
