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Outline
To investigate the differences in metabolic composition between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi）.
The volatile components of 66 batches of Daguomujiangzi（Fructus Cinnamomi） were qualitatively and quantitatively analyzed by supercritical fluid extraction（SFE -CO2） and gas chromatography-mass spectrometry（GC-MS），combined with cluster analysis（HCA），principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）.According to the characteristic coefficient of the overall variable VIP≥2，the differential metabolites between the genuine and non-genuine estate areas were screened.
The results of supercritical fluid extraction（SFE-CO2）of the volatile oil of Daguomujiangzi（Fructus Cinnamomi）showed that its content ranged from 18% to 33%，and the content of volatile oil in the genuine production area was significantly higher than that in the non-genuine production area.The results of volatile oil metabolomics showed that there were obvious differences between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi），among which 1，4，7，-Cyclo undecatriene，1，5，9，9-tetramethyl-Z，Z，Z-，beta-copaene，bicyclic dabanerene and terpenoids could be used as the metabolites for identifying the differences between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi）and the metabolism of Daguomujiangzi（Fructus Cinnamomi）can be classified into two categories，namely，the South Panjiang River，North Panjiang River and Yujiang River，along the sub-watersheds of Daguomujiangzi（Fructus Cinnamomi）.The composition pattern of the South Panjiang，North Panjiang and Yujiang rivers can be classified into three chemotypes.
There were significant differences in volatile component metabolites between the genuine and non-genuine production areas，which could provide certain reference value for the subsequent identification and quality evaluation of the genuine production areas of Daguomujiangzi（Fructus Cinnamomi）.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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