To study the changes in metabolites in platelets of a rat model of Qi deficiency and blood stasis syndrome using metabolomics methods and further elucidate the intervention effect of Buyang Huanwu Decoction（补阳还五汤，BYHWD）on Qi deficiency and blood stasis syndrome.

Thirty Sprague dawley（SD）rats were randomly divided into the normal，model and BYHWD groups. The model group established a sleep deprivation model through a multi-platform sleep deprivation system in the water environment. The BYHWD group was administered for two weeks after successful modeling，with sleep deprivation added simultaneously. The administration group was orally administered BYHWD at a clinical dose of 13 g· kg^-1，while the normal and model groups were orally administered an equal amount of distilled water once a day at the same time for 14 consecutive days. The construction of a comprehensive evaluation model and platelet function were evaluated from the perspectives of grip，coagulation function，platelet morphology，aggregation rate and CD62P（P-Selectin）expression rate. The metabolomics analysis of endogenous metabolites in rat platelets was carried out by using Ultra-high Performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry（UPLC-Q-TOF-MS）. The detection conditions were reverse phase chromatography column（RPLC）and Hydrophilic Interaction Chromatography（HILIC）chromatographic column，mobile phase 0.1% formic acid aqueous solution（A）-0.1% formic acid acetonitrile solution（B）gradient elution，flow rate 0.3 mL· min^-1，electric spray ion source（ESI），positive and negative ion mode detection，scanning range m/z50-1000. The multivariate statistical analysis was used to screen the inter-group differential ions and match fragment ion information with databases such as the Human Metabolome Database（HMDB）to identify biomarkers. The biological information of metabolites was obtained through the Kyoto Encyclopedia of Genes and Genomes（KEGG）database，and their related metabolic pathways were analyzed using MetaboAnalyst 5.0 software.

Study showed that compared with that of the normal group，the grip of the model group decreased（P<0.01），and BYHWD significantly increased the grip compared to the model group（P<0.01）. Compared with that of the normal group，the activated partial thromboplastin time（APTT）of coagulation function in the model group decreased（P<0.01），while the thrombin time（TT）increased（P<0.05）and the level of fibrinogen（FIB）increased（P<0.05）. Compared with those of the model group，the APTT in the BYHWD group significantly increased（P<0.01），while FIB level decreased（P<0.05）. Compared with those of the normal group，the maximum platelet aggregation rate（MAR）and average platelet aggregation rate（AAR）of the model group rats were significantly increased（P<0.01）. Compared with those of the model group，the MAR and AAR of platelets in the BYHWD group were significantly reduced，with varying degrees of difference（P<0.01，P<0.05）. Compared with that of the normal group，the expression of platelet CD62Pin the model group was significantly increased（P<0.05），and the BYHWD group could significantly reduce the expression rate of platelet CD62P（P<0.05）. The scanning electron microscopy results showed that compared with the normal group，the model group showed excessive platelet activation，significant pseudopodia extension and increased aggregation between platelets. Compared with the model group，the BYHWD group had fewer platelet activation and aggregation phenomena. The metabolomics results showed that the platelet metabolism of the rat model with Qi deficiency and blood stasis syndrome was disrupted，resulting in 95 significantly different metabolites. The main differential metabolites were concentrated in fatty acids，amino acids，phosphatidylcholine，lysophosphatidylcholine，nucleotides，etc. The intervention effect of BYHWD on Qi deficiency and blood stasis syndrome was mainly reflected in the regulation of metabolic pathways such as lipid metabolism，amino acid metabolism，nucleic acid metabolism and energy metabolism.

Study preliminarily reveals the metabolomics characteristics of platelets in rats with Qi deficiency and blood stasis syndrome and provides a basis for the mechanism of action of BYHWD on platelets in the intervention of Qi deficiency and blood stasis syndrome.