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Outline
To explore the effect of Wenyang Zhenshuai Granule（温阳振衰颗粒）medicated serum on adriamycin induced H9C2 myocardial cell injury based on long non-coding RNAs（LncRNA）metastasis associated lung adenocarcinoma transcript 1（malat-1）/microRNA-133（miR-133）pathway regulating p-ERK1/2.
H9C2 myocardial cell injury model was established by coculture with 2.67μmol/L adriamycin（ADR）for 45 h and set up a normal control group，a normal＋W group（W：Wenyang Zhenshuai Granule medicated serum），an miR-133 overexpression group，an miR-133 overexpression＋W group，an ADR group，an ADR ＋W group，an ADR ＋miR-133 overexpression group，and an ADR ＋miR-133 overexpression＋W group. The cell proliferative activity was detected by MTT method，while the change of N-terminal B-type brain natriuretic peptide precursor（NT-proBNP）was detected by ELISA. The contents of lncRNA malat-1，miR-133 and ERK1/2 mRNA in H9C2 cardiomyocytes of each group were detected by RT-PCR. The protein expressions of ERK1/2 and p-ERK1/2 were detected by Western-blot.
The myocardial cell activities in the ADR group，the ADR＋miR-133 overexpression group，the ADR＋W group，the ADR＋miR-133 overexpression＋W group，the normal control group，the miR-133 overexpression group，the normal＋W group and the miR-133 overexpression＋W group were increased in sequence（P<0.05）. The content of NT-proBNP was decreased in sequence（P<0.05）. After the experiment，there was no significant difference in ERK1/2 mRNA expression in myocardial cells among different groups（P>0.05）. The expression of the miR-133 in ADR group，the ADR＋miR-133 overexpression group，the ADR＋W group，the ADR＋miR-133 overexpression＋W group，the normal control group，the miR-133 overexpression group，the normal＋W group and the miR-133 overexpression＋W group increased in sequence（P<0.05）. The expression of LncRNA malat-1 was decreased in sequence（P<0.05）. There was no significant difference in ERK1/2 protein expression in myocardial cells among different groups after the experiment（P>0.05）. The p-ERK1/2 protein expression levels in the ADR group，the ADR＋W group，the ADR＋miR-133 overexpression group，the ADR＋miR-133 overexpression＋W group，the normal control group，the miR-133 overexpression group，the normal＋W group and the miR-133 overexpression＋W group were decreased in sequence（P<0.05）.
Wenyang Zhenshuai Granule medicated serum can reduce the expression of p-ERK1/2 protein in myocardial cells and exert the curative effect on chronic heart failure，which may be achieved by regulating the lncRNA malat-1/miR-133 pathway in myocardial cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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