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Outline
To study the effectof Ganfei Bingzhi Formula（肝肺并治方）on gutmicrobiota of ratswith ulcerative colitis.
Thirty SD ratswere randomly divided into Ganfei Bingzhi Formula high dose group（A），Ganfei Bingzhi Formulamedium dose group（B），Ganfei Bingzhi Formula low dose group（C），model group（D）and blank group（E），with 6 rats in each group. A ratmodel of UC was established by using trinitrobenzenesulfonic acid solution（TNBS）/ethanol method. After gavage with different intervention factors，the intestinal contents of rats were taken out and the bacterial population was sequenced using 16S rRNA method.
The dominant bacteria in the intestinal contents of rats in each group were Firmicutes and Bacteroidetes. Aftermodeling，the number of Firmicutes decreased，while the number of Bacteroidetes increased. Intervention with Ganfei Bingzhi Formula can increase the number of Firmicutes and reduce the number of Bacteroidetes. The effectof themedium dose group was themost significant. The sparse curve ofα-diversity tended to flatten out as the sequencing depth increased. The difference between themedium dose group and the blank group in the PCoA analysis chartand the distancematrix ofβ-diversity were the smallest. The composition of intestinal contents in themiddle dose group was themost similar to that of the blank group in phylum level，species level and genus level. There was a significant difference in the phylum of actinomycetes among the groups.
Ganfei Bingzhi Formula can correct the distribution of intestinalmicrobiota in rats with UC，making it tend to the normal gutmicrobiota. Among them，the effect of themedium dose group ismost obvious.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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