Full
Outline
To treat the rat model of chronic eczema by Sanzi Granules（三子颗粒）and explore its mechanism of external treatment for chronic eczema.
Forty male SD rats were randomly divided into normal group，model group，Sanzi Granules group and Qingpeng Ointment（青鹏软膏）group，with 10 rats in each group. The rat models of chronic eczema were prepared by sensitization with dinitrochlorophenylacetone solution. The model group was given wet compress with normal saline，the Sanzi Granules group was given wet compress with Sanzi Granules lotion，and the Qingpeng Ointment group was externally applied with Qingpeng Ointment. Skin lesion score was used to evaluate the curative effect. HE staining method was used to observe the morphological changes of the skin tissue. The expressions of interferon gamma（IFN-γ）and interleukin-4（IL-4）in skin lesions were detected by immunohistochemical technique. The protein levels of interleukin-17（IL-17），forkhead transcription factor 3（Foxp3），phosphorylated-p38 mitogen activated protein kinase（p-p38MAPK），p38 mitogen activated protein kinase（p38MAPK），phosphorylated-nuclear factor-κB p65（p-NF-κB p65）and nuclear factor-κB p65（NF-κB p65）in skin lesions were detected by Western blot.
The model group rats showed hyperkeratosis and dyskeratosis，with significant thickening of both the granular and spinous layers，and coarser collagen fibers. In the model group，the number of mast cells was significantly increased，the protein expressions of IFN-γ and Foxp3 were decreased，and the protein expressions of IL-4，IL-17，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65 were increased. Compared with those of the model group，the skin lesion scores of the Sanzi Granules group was significantly reduced on the 6th，8th and 10th day. The alleviated pathological changes and the protein expressions of IFN-γ and Foxp3 significantly increased（P<0.01），the protein expressions of IL-4，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65 were significantly reduced（P<0.05，P<0.01），while the expression of IL-17 decreased，but the difference was not statistically significant（P>0.05）.
Sanzi Granules can inhibit hyperplasia of mast cells，up-regulate the protein levels of IFN-γ and Foxp3，and reduce protein levels of IL-4，IL-17，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65，and play a therapeutic role in chronic eczema. These results preliminarily reveal the mechanism of Sanzi Granules treating chronic eczema.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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