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Outline
To exploring the enhancing effect of salt processing on the nourishing Yin and clearing heat effects of Dihuang（Rehmanniae Radix）.
Based on the ancient records of salt preparation method and modern evolution，the saltprocessed products of Dihuang（Rehmanniae Radix）were prepared. On this basis，a mouse model of Yin deficiency and internal heat induced by oral administration of levothyroxine sodium（160 mg· kg-1），which was relatively recognized and widely used，was studied by observing the gross signs，water intake，anal temperature，thermotropism as well as serum triiodothyronine（T3），thyroxine（T4），cyclic adenosine monophosphate（cAMP），cyclic guanosine monophosphate（cGMP），Na+-K+-ATP（Sodium Potas）and other factors to comprehensively evaluate the synergistic effect of salt processing on the main efficacy of nourishing Yin and clearing heat in Dihuang（Rehmanniae Radix）.
The model caused a significant increase in water intake，anal temperature，serum levels of T3，T4，cAMP，cAMP/cGMP，Na+-K+-ATPase and Ca2+-Mg2+-ATPase in mice（P<0.01），and the thermalization of the hot and cold board was significantly reduced（P<0.01）. Raw Dihuang（Rehmanniae Radix）and its various salt-processed products can significantly improve the abnormalities in the above indicators caused by the model（P<0.05 or P<0.01），among which the improvement effect of Dihuang（Rehmanniae Radix）salt-products was better than that of their raw products，and the effect of Dihuang（Rehmanniae Radix）salt-products processed by salt sealing freeze-drying method was particularly good.
The processing with salt can enhance the main effect of Dihuang（Rehmanniae Radix）in nourishing Yin and clearing heat. In particular，the enhancing effect of salt sealing freeze-drying processing method on the main effects of Dihuang（Rehmanniae Radix）is the best.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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