Full
Outline
To analyze the chemical composition and contents of Zhishi（Aurantii Immaturus Fructus）-Baizhu（Atractylodis Macrocephalae Rhizoma）essential oil（ZBEO）and explore the acute toxicity of different doses of chemical constituents of ZBEOin mice.
GC-MSwas used to analyze and identify the chemical composition. After oral administration of jojoba oil and ZBEO，the activity，diet，water intake，body weight，mortality and other conditions of the mice were observed. The values of median lethal dose（LD50）and 95% confidence interval were calculated by using the weighted regression method（Bliss）.
Through GC-MS analysis，a total of 10 chemical components were identified in ZBEO，accounting for 46.41% of the total oil content. The main components were curzerenone，D-limonene，β-pinene，γ-elemene，atractylenolide Ⅱ，β-elinene and trans-caryophyllene. The toxicity experiment of ZBEO on mice showed no significant effects on the diet，water intake or body weight of the surviving mice. The value of LD50 of ZBEO was calculated to be 1.681 mL/kg by using the Bliss method，indicating a certain level of toxicity. The 95% confidence interval for LD50 was 3.367～7.417 mL/kg. The regression equation was Y（Probit）＝-1.228+7.612 lgD.
Curzerenone and D-limonene are the main components of ZBEO. The essential oil exhibits a certain level of toxicity，mainly affecting the blood vessels as indicated by organ index and pathological observations. The main affected organs are liver，spleen and kidney. The toxicity is dose-dependent，and within a certain dosage range，it can be safely used.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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