Full
Outline
To observe the differences in biological traits between male and female Fangji（Stephania tetrandra）and to screen and identify simple sequence repeats（SSR）loci，so as to provide methods and basis for clarifying the differences bet ween male and female Fangji（Stephania tetrandra）and identifying the sexes of Fangji（Stephania tetrandra）in the early stage.
The differences in biological traitswere observed and analyzed by using the artificial ex-situ planting for 4 years as experimental materials，and the SSR marker identification technology was used to dig out and verify the specific SSR loci ofmale and female Fangji（Stephania tetrandra），and the SSR marker sites that can be used for early identification ofmale and female Fangji（Stephania tetrandra）were screened and obtained.
There was no significant difference in the phenotypic traits of female and male Fangji（Stephania tetrandra）at the 0.05 significant level，and there was no significant difference in the diameter，surface color or cross-section color of male and female Fangji（Stephania tetrandra），but the diameter，surface color or cross-section color of of female Fangji（Stephania tetrandra）were lighter and more brittle and powdery than those of male Fangji（Stephania tetrandra）. A total of 38 956marker sites were obtained by mining SSRmolecular marker sites with MISA，including 25 632 loci formale plants and 30 389 loci for female plants.
There were some differences in the phenotypic traits of male and female Fangji（Stephania tetrandra）and the traits of medicinal materials，but there was no significant difference in the overall quality traits. The SSR molecular marker differential loci screened in this study can identify the sex of Fangji（Stephania tetrandra）at an early stage，which can provide an effective basis for the development of Fangji（Stephania tetrandra）planting industry.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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