2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) is a natural product of chalcone type isolated from diverse plants with a variety of great pharmacological properties. This article reviews the research progress from physicochemical properties, sources, extraction and detection, chemical synthesis, and pharmacological activities, in order to provide a theoretical basis for the development or prodrug design of DMC.

Objective: To establish a quantitative proton nuclear magnetic resonance method for the determination of vemurafenib.Methods:The structure of vemurafenib was confirmed by MS and NMR. The spectra of proton nuclear magnetic resonance spectroscopy were obtained in dimethylsulfoxide-d₆ by using Bruker ANANCE NEO 600, the parameters of which were set up as follows: 90° pulse sequences, the relaxation delay time was 15 s, the number for scanning was 32, the experiment temperature was controlled at 25 ℃, and hydroquinone were selected as internal standard. The quantitative study was conducted on vemurafenib.Results:The ¹H and ¹³C NMR spectra of vemurafenib was assigned. The content of vemurafenib determined by qHNMR were 98.78%, which was basically consistent with the results of mass balance method (98.91%).Conclusion:The method is simple, rapid and accurate, and can be used for the determination of vemurafenib.

The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) Q11 is the technical guideline for the development and manufacture of drug substances, which systematically illustrates the principles of process development and selection of starting materials. ICH Q11 Q&A document further elaborates the specific requirements for the selection of starting materials. This paper discusses the consideration based on ICH Q11 in the technical review of chemical innovative drugs with respect to the requirements of ICH Q11 and the Q&A document, and further discusses the general considerations of ICH Q11 on the technical review of new drugs focusing on the periodic rules regarding pharmaceutical research of innovative drugs.

Objective: To evaluate the tolerability and pharmacokinetics of sitafloxacin fumarate injection in healthy Chinese volunteers.Methods:A total of 40 volunteers, half male and half female, were enrolled for single administration and multiple administrations of sitafloxacin fumarate injection. The single dose groups were further divided into 400 mg group and 500 mg group, and the tolerance was investigated. Multiple dose groups were divided into 300 mg (qd) group and 400 mg (qd) group to investigate the tolerability and pharmacokinetics. Adverse reactions were observed during the trial, and changes in vital signs, laboratory tests and other data were monitored to evaluate the safety and tolerability of the drug. The plasma concentrations of sitafloxacin were determined using HPLC-MS/MS, and its pharmacokinetic parameters were calculated and statistically analyzed.Results:No serious adverse events occurred during the trial. Main pharmacokinetic parameters of sitafloxacin after repeated administration are as follows: C_max, AUC_0-t, and AUC_0-∞ are (2 913.00±436.63) μg·L^-1, (18 368.93±4 141.28) h·μg·L^-1 and (19 662.17±4 820.31) h·μg·L^-1 in 300 mg (qd) group on day 1 of repeated administration; while (3 080.00±695.11) μg·L^-1, (22 625.89±5 729.43) h·μg·L^-1, and (22 990.68±5 866.94) h·μg·L^-1 on day 7. The corresponding values are (4 590.00±894.67) μg·L^-1, (24 113.91±5 030.12) h·μg·L^-1 and (25 277.71±5 159.69) h·μg·L^-1 in 400 mg (qd) group on day 1 of repeated administration; while (4 570.00±1 230.04) μg·L^-1, (30 280.20±7 007.20) h·μg·L^-1 and (30 586.30±7 055.85) h·μg·L^-1 on day 7.Conclusion:Sitafloxacin fumarate injection is safe and well tolerated in Chinese healthy volunteers. The dynamic characteristics of multipledoses were nonlinear, without significant accumulation in patients of once-daily administration. There was no statistical significance in gender difference of main pharmacokinetic parameters within the same dose group.

The concept of patient-focused drug development has been paid increasing attention by researchers in China and abroad. Patient-reported outcome (PRO) has gradually become one of the endpoints in clinical trials. PRO provides health information directly from the patient and plays an important role in the clinical decision making by physicians. However, the use of PRO assessment and data interpretation in oncology clinical trials are still waiting for further improvement. The selection of core PRO indicators is critical to the consistency of PRO assessment criteria. In 2021, U.S. FDA issued the "Core Patient-Reported Outcomes in Cancer Clinical Trials Guidance for Industry (draft)" for drug development in cancer clinical trials, providing guidance for sponsors on core PRO collection, PRO assessment tool selection, and standardized trial design. This paper interprets the systematic assessment and application of core PRO in the guideline, helps practitioners of new drug clinical trials to further understand PRO and promotes the implement of PROs in new drug clinical trials in a good way.

Objective: To eatablish a UPLC-MS/MS method for determination of three azide-type genotoxics, 5-(4′-(azidomethyl)-[1,1′-biphenyl]-2-yl)-1H-tetrazole (MB-X), 4′-(azidomethyl)-[1,1′-bihenyl]-2-carbonitrile (AZBC), and 5-(4′-((5-azido-2-butyl-4-chloro-1H-imidazol-1-yl)methyl)-[1,1′-biphenyl]-2-yl)-1H-tetrazole (LADX), in active pharmaceutical ingredients (API) of irbesartan and losartan potassium.Methods:The separation was performed on a ACQUITY UPLC HSS T3 column(100 mm×2.1 mm, 1.8 μm) with the mobile phases consisting of 0.1% formic acid aqueous solution (mobile phase A) and 0.1% formic acid methanol solution (mobile phase B) using a gradient elution at a flow rate of 0.35 mL·min^-1. The column temperature was set at 50 ℃. Multiplereaction monitoring (MRM) was performed on a triple quadripole mass spectrometer equipped with a APCI source in positive/negative mode.Results:the calibration curve was linear for three compounds in the range of 0.5~100 ng·mL^-1. The recoveries (n=3) in irbesartan of low, middle, high concentration-spiked samples are 94.5%~103.5% with RSD<3.88%. The recoveries (n=3) in losartan potassium of low, middle, high concentration-spiked samples are 93.8%~100.9% with RSD<4.25%. The limits of detection are 0.05, 0.03, 0.02 ng·mL^-1 and the limits of quantification are 0.15, 0.11, 0.08 ng·mL^-1, respectively.Conclusion:The method is sensitive and accurate, which is applicable for quantification of three azide-type genotoxic impurities in irbesartan API and losartan potassium API. The method can provide reference for quality control of irbesartan and losartan potassium.

Cichorium glandulosum is a special medicinal and edible plant in Xinjiang of China, which contains polysaccharides, flavonoids, terpenoids as the main active ingredients. The significant pharmacological activities include liver protection, lipids reduction, anti-inflammatory and so on. It is a traditional medicine commonly used by people of all ethnic groups in Xinjiang of China. C.glandulosum was searched as the key word in CNKI, WANFANG and PubMed database as the search platform, using search items of keywords and full text. The research hotspots of C.glandulosum was statistically analyzed in recent years. The paper reports and patent of C.glandulosum were retrieved, 100 papers and 15 patents were included, among which studies of pharmacognosy, chemical composition, and pharmacological activity were involved, and the patents mainly relate to its biomedicine, health care products, and preparation techniques. In this paper, cultivation, pharmacognosy, pharmacological activities, and patent application of C.glandulosum are reviewed and prospected, in order to provide reference for the development and utilization of C.glandulosum. It is suggested that C.glandulosum had potential research value in medicine, health care products, food and other industries.

Objective: Three kinds of paclitaxel saturated fatty acid ester liposomes were prepared to preliminarily compare the difference between them in anti-tumor, and to explore the reasons for the difference of efficacy by the study of tissue distribution.Methods:Three kinds of paclitaxel fatty acid ester liposomes were prepared by thin film dispersion method, and the particle size, potential, encapsulation efficiency and drug loading were measured, respectively. A mouse model of breast cancer was established, and paclitaxel injection (Taxol), paclitaxel myristate liposome (PTX-MA-L), paclitaxel palmitate liposome (PTX-PA-L) and paclitaxel stearate liposome (PTX-SA-L) were injected via the tail vein, respectively. The anti-tumor effects of the two preparations were investigated, and the safety of the preparations was evaluated by routine blood tests. Finally, the prodrug metabolism for three prodrug liposomes was evaluated by in vivo tissue distribution.Results:Three taxol saturated fatty acid ester liposomes were successfully constructed. The particle sizes of PTX-MA-L, PTX-PA-L and PTX-SA-L are (69.56±1.08), (72±0.86) and (75±1.02) nm; Polymer dispersity index (PDI) are (0.185±0.009), (0.165±0.012), (0.171±0.024); Zeta potentials are -(11.26±1.37), -(12.18±1.86), -(13.92±0.59) mV, respectively. Encapsulation rates are (95±0.39)%, (97±0.28)% and (98±0.19)%, respectively. In pharmacodynamic evaluation in 4T1 breast cancer transplanted tumor model, the pharmacodynamic of PTX-PA-L at equal dose was stronger than that of taxol, PTX-MA-L and PTX-SA-L. The results of routine blood test showed that the blood cell level of paclitaxel fatty acid ester liposome was significantly higher than that of paclitaxel injection. The tissue distribution results showed that PTX-MA-L was metabolized to PTX at a fast rate, short half-life and fast elimination rate. PTX metabolized from PTX-PA-L increases slowly with time. Its half-life is the longest among the three prodrug liposomes, the area under the drug-time curve is the largest, and the clearance rate is the lowest. PTX-SA-L was metabolized to PTX the most slowly, and the maximum plasma concentration was the lowest in tumors.Conclusion:The paclitaxel prodrug liposome modified with saturated fatty acid has better tumor treatment effect and alleviates the toxic and side effects caused by traditional paclitaxel dosage forms. Among them, the antitumor effect of PTX-PA-L is better than that of PTX-MA-L and PTX-SA-L, which may be related to the metabolism of fatty acid prodrugs in vivo.

Objective: To establish thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) characteristic chromatography methods of 70% ethanol extract from seeds of fenugreek, and to investigate the similarities and differences among eleven batches of fenugreek extract.Methods:① G and HF₂₅₄ silica gel plates were used for TLC, using a mixture of n-butanol-ethanol-water (2∶6∶2) as developer, bismuth potassium iodide-ferric chlorideand and 10% sulphuric acid-ethanol as color developing reagents. The sunlight and ultraviolet wavelength at 254 nm and 365 nm were used to identify the spots on TLC plats. ② A Supersil ODS-B column (250 mm×4.6 mm, 5 μm) was used for HPLC chromatography. The mobile phase was 0.1% phosphoric acid-acetonitrile with a gradient elution. The flow rate was 0.7 mL·min^-1, the column temperature was 30 ℃, and the detection wavelength was 203 nm.Results:The TLC chromatogram of 70% ethanol extract from fenugreek indicated that there were eight spots containing trigonelline and dioscin as anti-diabetic active components. In HPLC chromatogram, there were twenty-nine common peaks in eleven batches of fenugreek, and fourteen components were identified. The similarity of the eleven batches of 70% ethanol extract were more than 0.9. The cluster analysis showed that there was variability among the samples of fenugreek from different origins. However, there are high similarity between Anhui and Henan provinces.Conclusion:These developed methods are reproducible and reliable. The two chromatograms showed strong characteristics, which can be used to control the quality of 70% ethanol-extract of fenugreek.

Objective: To systematic review the efficacy and safety of ceftazidime avibactam (CAZ/AVI) and colistin/polymyxin B in the treatment of carbapenem-resistant Gram-negative bacilli infection.Methods: Cochrane Library, PubMed, Embase, CNKI and Wanfang data were searched to collect randomized controlled or cohort studies of ceftazidime avibactam and colistin/polymyxin B in the treatment of carbapenem-resistant Gram-negative bacilli infection. The retrieval time was from the establishment of each database to May 2022. After literature screening and data extraction, NOS was used to evaluate the quality of literature.Results: Totally 7 studies were finally enrolled, with a total of 801 patients. The results of Meta-analysis showed that the mortality rate and incidence of kidney injury in CAZ/AVI group were lower than those in colistin/polymyxin B group (P<0.005), while the clinical cure rate and bacterial clearance rate were higher than those in colistin/polymyxin B group (P<0.005).Conclusion: The clinical efficacy and safety of CAZ/AVI in the treatment of carbapenem-resistant Gram-negative bacilli infection showed better efficacy and lower incidence of kidney injury compared with the control group.