PDF
Full
Outline
Objective: To establish thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) characteristic chromatography methods of 70% ethanol extract from seeds of fenugreek, and to investigate the similarities and differences among eleven batches of fenugreek extract.Methods:① G and HF254 silica gel plates were used for TLC, using a mixture of n-butanol-ethanol-water (2∶6∶2) as developer, bismuth potassium iodide-ferric chlorideand and 10% sulphuric acid-ethanol as color developing reagents. The sunlight and ultraviolet wavelength at 254 nm and 365 nm were used to identify the spots on TLC plats. ② A Supersil ODS-B column (250 mm×4.6 mm, 5 μm) was used for HPLC chromatography. The mobile phase was 0.1% phosphoric acid-acetonitrile with a gradient elution. The flow rate was 0.7 mL·min-1, the column temperature was 30 ℃, and the detection wavelength was 203 nm.Results:The TLC chromatogram of 70% ethanol extract from fenugreek indicated that there were eight spots containing trigonelline and dioscin as anti-diabetic active components. In HPLC chromatogram, there were twenty-nine common peaks in eleven batches of fenugreek, and fourteen components were identified. The similarity of the eleven batches of 70% ethanol extract were more than 0.9. The cluster analysis showed that there was variability among the samples of fenugreek from different origins. However, there are high similarity between Anhui and Henan provinces.Conclusion:These developed methods are reproducible and reliable. The two chromatograms showed strong characteristics, which can be used to control the quality of 70% ethanol-extract of fenugreek.
PDF
34
14
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
