[Objective] In August 2023,an outbreak of infectious disease characterized by respiratory symptoms was reported on one cattle farm in Tianjin. To determine the cause of the disease,we conducted etiological diagnosis of the relevant pathogens in the affected calves. [Method] Lung specimens were collected from dead calves. We used the PCR method to detect seven bovine respiratory pathogens,including Bovine parainfluenza virus type 3,Bovine respiratory syncytial virus,Bovine herpesvirus type 1,Bovine adenovirus type 3,Mycobacterium bovis, Bovine Klebsiella bovis and Mycoplasma bovis. [Result] The results showed that two pathogens,BHV-1 and BAdV-3,were detected in the samples,and there was mixed infection. No other pathogens were detected. [Conclusion] This study confirmed the existence and prevalence of BHV-1 and BAdV-3 in dairy cows in this cattle farm,which provided reference for the prevention and control of respiratory diseases.

[Objective] Lactic acid bacteria are of great significance in promoting human health. As good carriers, fermented milk and live lactic acid bacteria beverage are important sources of lactobacillus supplementation for human body. The national standards clearly specifies the quantity of lactic acid bacteria in fermented milk and live probiotic beverages, so it is important to ensure the accuracy of detection results. [Method] Common laboratory medium are selected as the interference components, and the effects of various interfering components on lactic acid bacteria culture media and national standard test results are explored through artificial addition.[Result] The total number of bifidobacterium is the most affected by rose Bengal medium and BS medium, and the least affected by VRBA medium. The total number of Streptococcus thermophilus is the most affected by TTB medium and the least affected by VRBA medium. The total number of Lactobacillus is most affected by SC medium, VRBA medium and XLD medium have the least effect on Lactobacillus. MRS AGAR medium is most affected by SC medium composition, modified MRS AGAR medium is most affected by SC medium, MC AGAR medium is mainly affected by high supplemental BS medium. [Conclusion] The results provided a basis for judging the culture status of lactic acid bacteria through the medium, colony sense and colony number changes, and provided a reference for the abnormal analysis of lactic acid bacteria detection results, and have certain guiding significance for ensuring the accuracy of lactic acid bacteria detection results.

In the context of the digital economy,the practical significance of dairy brand building mainly lies in enhancing market influence and competitiveness,increasing sales revenue and operating profits,and building a responsible social image for the enterprise. Taking Xueyuan Dairy as an example,its brand building is facing problems such as the lack of effective digital tools and platforms,low integration of online and offline,insufficient consumer participation,and unclear brand stories and value transmission.To enhance brand building,it is recommended that Xueyuan Dairy strengthen its digital infrastructure construction,establish an online and offline linkage mechanism,and clarify its brand value positioning to help Xueyuan Dairy achieve comprehensive brand building in the digital economy era.

The effect of the transition from conventional milking system to milking robots varies from dairy farm to dairy farm,mainly due to the differences in the start-up process and the preparedness for milking robots on dairy farms, amongst many other reasons.This paper described and summarized the important factors affecting the start-up of milking robots in dairy farms from different perspectives such as hardware,different labor,and management for the enablement of milking robots.

Since 2017,Yishui County has continued to promote whole corn silage technology. The application of whole corn silage in cattle raising in Yishui County was analyzed by summarizing the data over the years.Visiting the cattle farm to know the whole corn silage situation on site.The disadvantages and key points of whole maize silage popularization and application were studied.The main points and economic benefits of whole plant corn silage were summarized,and the experience was summarized to provide reference for the breeding enterprises using whole corn silage and the relevant animal husbandry departments promoting this technology.

[Objective] To study the effects of adding bacterial enzyme preparations to dairy cow diet on blood biochemical and immune indexes.[Method] A total of 200 healthy lactating cows with similar age and parity in a large-scale dairy farm were selected as the research objects and divided into 4 groups（n=50） by random number table method.They were divided into control group（basic diet）,bacterial enzyme preparation group 1（basic diet+0.1% bacterial enzyme preparation） and bacterial enzyme preparation group 2（basic diet+0.2% bacterial enzyme preparation） and bacterial enzyme preparation group 3（basic diet+0.3% bacterial enzyme preparation ）. Each group was set with 5 replicates and 10 lactating cows per replicate.The pre-test period lasted for 7 days and the test period lasted for 42 days.The blood biochemical and immune indexes of lactating cows in each group were analyzed statistically.[Result] ALT,TBIL,CK,ALB,BUN,Na,P,Ca and other blood biochemical indexes of lactating cows in bacterial enzyme preparation group 1,group 2 and group 3 were better than those in control group（P<0.05）. The blood biochemical indexes of bacterial enzyme preparation group 2 were the best.There was no significant difference in IgM,IgG and TNF-α in control group,bacterial enzyme preparation group 1,group 2 and group 3（P> 0.05）. IgA in bacterial enzyme preparation group 2 was higher than that group 1,group 3 and control group（P<0.05）,and there was no significant difference between bacterial enzyme preparation group 1 and group 3（P>0.05）.IL-2 in bacterial enzyme preparation group 1,group 2 and group 3 were higher than that in control group（P<0.05）,and there was no significant difference in IL-2 in group 1,group 2 and group 3（P>0.05）.[Conclusion] Bacterial enzyme preparation could improve the blood biochemical indexes of lactating cows and enhance the immune capacity of the body,and 0.2% was the most suitable addition.

Infant formula is the only or primary source for infants and young children to obtain part or all of the nutrition. Specific formulations should meet different nutritional needs,and ensuring the quality and safety of infant formula is related to the health of the next generation.In this paper,the key risks affecting the safety of infant formula,including the monitoring results of supervision and sampling in recent years,the report of relevant public opinion affairs and potential risks were comprehensively analyzed.Risk control measures were proposed for the entire industrial chain and supervision related links of production enterprises. In order to continuously improve the risk detection ability of infant formula,this paper provided scientific suggestions for promoting food supervision and sampling work and high-quality development of the infant formula industry.

Nutrition label of prepackaged food is important instructions for food nutrition information in prepackaged food label,which is of great significance to guide consumers to choose products reasonably. Dairy products are rich in nutrients, and it is more important to correctly label their nutritional information. Due to inadequate understanding or deviation of relevant standards by food production enterprises, there may be errors or defects in the labeling of nutrition information. This article takes dairy products as an example to summarize common problems in nutrition label of prepackaged food, propose correct label methods and compliance measures, and provide reference for dairy and food production enterprises.

With the development of large-scale dairy farms, manure generated during the breeding process and its treatment have become an important issue for the carbon reduction of agriculture and livestock. In order to solve this problem, taking Hanting District of Weifang City as an example, this paper introduced the mechanism,operation method and application effect of biogas fermentation technology and aeration fermentation technology in order to provide reference for dairy farm. Research showed that both technologies can achieve zero emissions of dung on dairy farms, increase the economic benefits of dairy farming, and promote the healthy and sustainable development of dairy industry. Biogas fermentation technology requires a large area and investment funds, and its effectiveness is better in summer and less in winter. Aerobic fermentation technology does not require a large area of land and invests less money than biogas fermentation technology. It can treat cow manure harmlessly in a short time, solve the problem of inefficiency caused by the excessive consumption time of biogas fermentation, and solve the problem that biogas fermenting cannot continue to operate because the output of gas is greatly reduced in winter. It is suitable for various large, medium and small dairy farms.

With the rapid development of the dairy industry,the quality and safety of dairy products have received much attention,and accurate and efficient detection technology has become the key.As the core equipment of modern dairy testing,the constant temperature and oscillation detection device integrates advanced temperature control and oscillation technology,which can simulate complex environment,ensure uniform heating and full reaction of samples,and provide accurate and reliable support for dairy testing. In this paper,the application of the device in dairy detection was discussed,including microorganism,nutrient composition,physical and chemical properties and pollutant detection.Its working principle,key technology and structure are described in detail. Comprehensive analysis of temperature control accuracy,oscillation performance,stability,sealing and other performance indicators.It also look forward to the future development trend from the aspects of innovative design, technology integration,standardization and intelligence,aiming to provide theoretical and technical support for improving the level of dairy testing and promoting the development of the industry.