To understand the HIV infection risk perception bias and its related influencing factors among young men who have sex with men (MSM) in Shandong Province, so as to provide reference for making feasible HIV health education plans.

From April to July, 2022, young MSM were recruited in six cities in Shandong province, and a questionnaire survey was conducted and blood samples were collected to collect demographic characteristics, HIV risk perception and risk behavior of MSM. Logistic regression model was used to analyze the influencing factors of HIV infection risk perception bias among young MSM.

A total of 978 MSM were investigated, of which 85%(831/978) were over 20 years old, 97.6%(955/978) were unmarried/divorced or widowed, 61.1%(598/978) were college educated, 2.6% (25/978) were positive for HIV antibody and 3.6%(35/978) for syphilis antibody. 47.1%(461/978) had HIV infection risk perception bias, of which 54.0%(249/461) underestimate the risk and 46.0%(212/461) overestimate the risk. Logistic regression analysis showed that unmarried/divorced/widowed (OR=11.693,95%CI:1.546-88.407) and having no or low risk of new drugs (OR=2.041,95%CI:1.477-2.825) were the risk factors for underestimating the risk. Household registration in Shandong province (OR=2.618,95%CI:1.397-4.902), college education or above (OR=1.728, 95%CI:1.206-2.475), homosexuality (OR=2.532, 95%CI:1.093-5.845), having general or high risk of new drugs(OR=8.323, 95%CI:4.602-15.052), who had not received AIDS intervention (OR=2.288,95%CI:1.224-4.292) and had not taken preventive drugs before exposure (OR=11.905,95%CI: 3.663-38.462) were the risk factors for overestimating the risk.

HIV infection risk perception bias among young MSM in Shandong Province is high, and HIV risk behaviors are widespread. It is necessary to strengthen the education of MSM who have low self-rated HIV infection risk but have high-risk behaviors, improve risk awareness and reduce the occurrence of dangerous behaviors.

To provide a basis for formulating precise prevention and control strategies, the temporal and spatial aggregation characteristics of county road traffic injury mortality were analyzed, Shandong, 2012-2021.

The traffic injury mortality rate was calculated by using the data from the death registration and reporting system of Shandong. Geoda 1.18 and SatScan 9.7 were used for spatial autocorrelation analysis and spatiotemporal scanning analysis to explore the characteristics of spatiotemporal aggregation, and principal component regression analysis was used to explore the influencing factors.

Road traffic injury mortality rate in Shandong from 2012 to 2021 showed an overall decreasing trend and was high in the fall. The spatial correlation existed in each year, and the "high-high" aggregation area was mainly located in the central and northwestern parts of Shandong. The spatial scan revealed the existence of four clusters across the entire population. Cluster 1 existed from March 2012 to February 2015, covering 26 districts and counties, primarily located in south-central Shandong. Similar to the total population, urban and rural areas were mainly located in the central and southern part of Shandong, and the main urban agglomeration area existed from January 2012 to December 2014, covering 19 districts and counties; The main rural agglomeration area existed from January 2012 to December 2014, covering 38 districts and counties. Principal component regression analysis showed that the variables reflecting the economic and transportation conditions had a greater impact. Among them, the number of permanent residents and the volume of passenger and freight transportation were positively correlated with the mortality rate, while others were negatively correlated.

From 2012 to 2021, the mortality rate of road traffic injuries in Shandong showed a decreasing trend. The main agglomeration area exists in the central and southern part of Shandong, and the rural agglomeration is more obvious. According to the characteristics of clustering, attention should be paid to the enforcement of laws and regulations, infrastructure construction, population flow and other factors in high-risk areas, and prevention and control strategies should be adopted or adjusted according to local conditions.

To analyze the trend of acute myocardial infarction mortality and potential life loss among residents in Nanning City from 2015 to 2023, and provide a basis for formulating chronic disease prevention and control policies.

To collate the death cause monitoring data of the whole population in Nanning City. Crude mortality, standardized mortality, age-specific mortality, composition ratio, potential years of life lost ( PYLL%), standardized years of life lost (SPYLL%) and average years of life lost (AYLL) were calculated.Joinpointlog-linear regression model was used for trend analysis.

From 2015 to 2023, a total of 38 870 deaths from acute myocardial infarction were reported in 12 counties (cities, districts) of Nanning City, including 22 566 males and 16 304 females. The crude mortality rate was 59.84/100 000, and the standardized mortality rate was 53.70/100 000. The crude mortality rate and standardized mortality rate both showed a stable trend, with AAPC values of -1.408 and -2.892, and P values of 0.443 and 0.054, respectively. The mortality rate increases with age and shows a linear increase after the age of 40. The proportion of pre hospital mortality in acute myocardial infarction ranged from 81.53% to 87.29%, which was at a high level, but showed an overall downward trend (AAPC=-0.552%, P=0.007). The potential life loss rate ranged from 0.33% to 0.42%, with an average life loss of 6.02 years to 7.03 years, and the trend of change was not statistically significant. However, the potential life loss rate and average life loss of women showed a downward trend, with AAPC values of -5.941% and -3.475%, respectively, and P values of 0.01.

The mortality rate of acute myocardial infarction in Nanning City is relatively high, and the potential loss of life is severe. Pre hospital emergency treatment and health education for acute myocardial infarction should be strengthened, and the elderly and men are the key population for prevention and con.

To use neural networks and optimization algorithms, establish an obesity level prediction model to assess obesity risk.

Perform correlation analysis on 2 111 recorded data collected from participants aged between 14 and 61 years old in Mexico, Peru, and Colombia, and establish a BP neural network obesity level prediction model. At the same time, optimize the number of hidden nodes and transfer function of the model through pruning to find the optimal network structure. In addition, the genetic algorithm and the simulated annealing algorithm were used to optimize the weights and thresholds of the model, ultimately establishing a high-precision and practical GASA-BP neural network obesity level prediction model.

The R² of the prediction model was 0.975 1, and the MAE was 0.352, indicating high prediction accuracy and strong practicality. In the process of predicting obesity levels in the model, weight index was the most important, with a correlation of 0.913 with obesity levels. The correlation between overweight members in the family was also relatively strong, with a correlation of 0.505.

The GASA-BP neural network prediction model performs better than other models in predicting obesity levels, and can make the most accurate prediction of obesity levels, providing guidance and reference for personalized obesity assessments and subsequent prevention and control measures.

To analyze the trends in incidence of acutemyocardialinfarction(AMI)in Wuhan from 2016 to 2023, and to provide scientific basisfor prevention and control of AMI in Wuhan.

The incidence data during 2016—2023 from the Surveillance System of cardiovascular diseases in Wuhan were collected to compute the incidence, age-standardized rate by China population 2010 (ASR-C) and by Segi’s world standard population(ASR-W).Theannual percent change (APC) and average annual percent change (AAPC) of incidence of AMI were evaluated using Joinpoint regression model,to analyzethe incidence trends of AMI.

The incidence of AMI in Wuhan increased from 56.67/10⁵ in 2016 to 115.29/10⁵ in 2023(APC=10.24%,P<0.05);andASR-C of AMI increased from 40.45/10⁵ in 2016 to 79.57/10⁵ in 2023(APC=9.75%,P<0.05).The AMI incidence of males(83.65/10⁵) was significantly higherthanthat of females(34.99/10⁵) from 2016 to 2023 (P<0.05).The AMI incidenceofurban area(62.59/10⁵) was significantly higher than that of rural area(55.35/10⁵)(P<0.05).The regression model showed that the incidence and ASR-C of AMI in male and female all showed a significant upward trend (all P<0.05);the incidence and ASR-C of AMI of urban residents all showed a significant upward trend (all P<0.05), while the trends of rural residents from 2016 to 2020 were not statistically significant, but they all showed a significantupward trend from 2020 to 2023 (all P<0.05).The incidence of AMI in age groups<30,30-<40,40-<50, 50-<60, 60-<70 years and≥70 years in Wuhan increased at different rates from 2016 to 2023(P<0.05).Incidence in agegroups 30-<40,40-<50 years increased rapidly,with the AAPCs of 16.73% and 13.87%, respectively.

The incidence of AMI in Wuhan showedan upward trend from2016 to 2023,young adult males are the crucial population for prevention andcontrol.

To investigate the epidemiologic profile of contamination by multiple respiratory viruses (including influenza virus, rhinovirus, adenovirus, and respiratory syncytial virus) in the environments of medical institutions in Changsha City in 2023, aiming to provide scientific evidence for the formulation of infection control measures.

A total of 663 environmental samples were collected from 11 medical institutions in Changsha City, encompassing various types such as air, desktops, countertops, trash cans, power switches, door handles, elevator buttons, faucets in washrooms, water dispensers, and other surface swab samples. RT-PCR technology was employed to conduct nucleic acid testing for multiple respiratory viruses. The overall positive rates of respiratory viruses among different types of medical institutions, surveillance areas, and environmental samples were comparatively analyzed.

The study revealed that the overall positive rate of nucleic acids for multiple respiratory viruses in the medical institution environments of Changsha City in 2023 was 10.41%, and the positive rates for influenza virus, rhinovirus, adenovirus, and respiratory syncytial virus were 0.90%, 5.28%, 3.77%, and 0.45%, respectively. Among the different types of medical institutions, general hospitals exhibited the highest positive rate of multiple respiratory viruses, reaching 15.22%, while specialized hospitals and other medical institutions (including welfare hospitals and physical examination institutions) had positive rates of 10.71% and 2.61%, respectively. In terms of surveillance areas, outpatient diagnosis and treatment areas demonstrated the highest overall positive rate of multiple respiratory viruses, standing at 12.50%, while public areas and inpatient wards exhibited lower positive rates. Additionally, swab samples collected from desktops, countertops, and trash cans showed the highest overall positive rate of multiple respiratory viruses, accounting for 15.45%. Samples from other sources, including faucets and water dispensers in washrooms, power switches, elevator buttons, door handles, and air samples, also exhibited varying degrees of positive rates. Statistical analysis demonstrated significant differences in the overall positive rates of multiple respiratory viruses among different types of medical institutions, surveillance areas, and environmental samples (P<0.05).

The presence of multiple respiratory viruses in the environments of medical institutions in Changsha City in 2023 suggests the urgent need for enhanced epidemiologic surveillance and disinfection measures to effectively prevent and control nosocomial infections.

To understand intake frequency of sugar-sweetened beverages (SSBs) and the consumption habit among primary and secondary students. To explore the relationship of SSBs cognition and behavior，and to provide a basis for reducing students’ intake of SSBs.

A multi-stage sampling process was employed to select subjects from primary and middle school in Shenzhen. Network questionnaire survey was conducted to collecting information in September and October, 2021. Spearman rank correlations was used to analyze relationships of various types of SSBs consumption frequency. Multivariable linear regression model was used to examine the relationship between SSBs cognition score and consumption frequency score. Hierarchical analysis to explore the interactions.

A total of 16 840 primary and secondary students aged 6 to 18 years old were included. The prevalence of SSBs consumption at least 3 time/week was 58.9%, the grades 1-3, 4-6 and middle school students were 52.3%, 60.3% and 67.6%. Parents (73.3%) were the main buyers of SSBs, convenience stores (73.7%) were the main places and delicious (77.2%) was the major cause of choices. There was a positive correlation between the intake frequency of different types of SSBs (P<0.001). The results of multivariate linear regression showed that the higher the cognition was, the lower the intake frequency of SSBs was (β=0.179, 95%CI：0.155-0.204). There was a multiplicative interaction between the cognitive score of SSBs and the educational level of parents (P<0.00l).

The intake frequency of SSBs was high among primary and middle school students in Shenzhen, and the cognitive level of SSBs was related to the intake frequency and the educational level of parents.

To investigate the dynamic developmental trajectories of early suicidal ideation among adolescents, and the influence of factors such as gender, left-behind status, only-child status, boarding status, and social support on the trajectories of suicidal ideation development.

A cluster sampling method was employed to conduct three follow-up surveys among 989 7th-grade students in a certain urban middle school in Hunan Province from March to December 2023. The adolescent suicidal ideation scale (PANSI) and adolescent social support scale (CASSS) were used for questionnaire surveys. A latent growth mixture model (LGMM) was utilized to analyze and explore different categories of early suicidal ideation among adolescents.

Four potential categories of developmental trajectories of early suicidal ideation among adolescents were identified, labeled as C1 group "high-risk-stable" (7.2%), C2 group "medium-risk-worsening" (8.8%), C3 group "medium-risk-stable" (11.1%) and C4 group "low-risk-stable" (72.9%). Being female, left-behind, and only-child were identified as risk factors for the developmental trajectories of early suicidal ideation (all P<0.05), while social support was identified as a protective factor for these trajectories.

The developmental trajectories of early suicidal ideation among adolescents exhibit significant heterogeneity among groups and individual differences.

To explore the status quo of breastfeeding social support in puerpera and analyze its influencing factors.

A total of 535 puerpera were selected from 4 community health service centers in Chengde city by complete random cluster sampling method.The random forest model and LASSO regression were used to rank and screen the influencing factors.

The total score of the Breastfeeding Social Support Scale was 52.00 (9.00). The results of LASSO regression showed that when the lambda(λ) value was 0.117 7, the error was the smallest, and the corresponding number of influencing factors was 10.The importance ranking and multiple regression analysis showed that the main influencing factors of breastfeeding social support were community promotion of breastfeeding, whether returning to work or not, employment status, whether breastfeeding at night or not, feeding patterns (P<0.05).

The level of breastfeeding social support remains to be improved,and health care providers should develop targeted intervention according to the influencing factors.

To understand the status quo of children’s obesity health literacy among parents of primary school students in Yangzhou City, analyze related influencing factors, and put forward new ideas for optimizing health education of childhood obesity prevention and control.

Stratified cluster random sampling was used to randomly select 1 street from each of the 6 counties (cities and districts) in Yangzhou City, and then randomly select 4 primary schools from each street, and randomly select at least 1 class from each grade (grades 1-6) in each primary school. Questionnaire survey was conducted on parents of the students in the whole class.

A total of 6 097 parents of primary school students were surveyed, among whom 3 615 reached the standard of health literacy, the rate of reaching the standard was 59.29%. The compliance rates of health cognition, health behavior, health knowledge, operation skills and health consciousness were 66.54%, 41.68%, 69.99%, 44.89% and 32.97%, respectively. According to χ² test, there were significant differences in parents’ health literacy attainment rates among different places of residence, age, occupation, education level, family income, relationship with children, and student weight status (all P values <0.05). Multivariate logistic regression analysis showed that residence, educational level, occupation and students’ weight status were the influencing factors of parents’ childhood obesity health literacy. Living in towns and villages (OR=1.223, 95%CI: 1.092-1.369) , profession of production/manufacturing/repair industry (OR=1.353, 95%CI: 1.059-1.729) and jobless person (OR=1.514, 95%CI: 1.126-2.036) or others (OR=1.404, 95%CI: 1.112-1.773), and students’ weight status as obesity (OR=1.203, 95%CI: 1.060-1.366) are risk factors for childhood obesity health literacy of primary school parents, and parents’ education of high school or above (OR=0.265-0.649, 95%CI: 0.133-0.752) are protective factors for childhood obesity health literacy of primary school parents.

The parents of primary school students in our city have a high level of obesity health literacy, but a low rate of health behavior, operation skills and health awareness. In the process of child obesity prevention and control education, it is necessary to pay attention to the parents of students living in towns and villages, with low education level, unemployment and parents of obese students, and carry out targeted health education activities.