Objective To establish a chemiluminescence immunoassay (CLIA) for the detection of serum thyroid function. Methods The clinical data of 121 patients with hyperthyroidism, 101 patients with hypothyroidism and 67 patients with normal thyroid function admitted to Tianjin Medical University General Hospital were retrospectively analyzed from February 2023 to May 2024. The levels of serum thyroid stimulating hormone (TSH), free thyroxine (FT4) and free triiodothyronine (FT3) were examined by CLIA, and then the linear range, precision and accuracy of TSH, FT4 and FT3 monitored by CLIA system were counted. Results The linear correlation coefficient(r)values of TSH, FT4 and FT3 were$\geq {0.990}$, and slope(b)was within 0.95-1.00. The intra-batch and inter-batch precision values of TSH, FT4 and FT3 were less than 1/4TEa (CLIA’88) and 1/3TEa (CLIA’88) respectively. The bias of TSH, FT4 and FT3 were less than 1/2TEa (CLIA’88). The levels of TSH, FT4 and FT3 in patients with hyperthyroidism, hypothyroidism and normal thyroid function detected by CLIA were higher than those detected by RAI (P<0.05). Conclusion CLIA has good accuracy and precision in the detection of three items of serum thyroid function, and provides an important basis for clinical detection of three indexes of serum thyroid function.

Objective To evaluate the advantage clinical value of cold digestion method and national standard method in the determination of serum iodine. Methods The DAT-500 iodine detector of Changsha Seclude and the cold digested serum iodine detection reagent (patent: CN113340888A reagent kit and detection method for serum quantitative detection) were used to compare with the national standard Determination of Iodine in Serum by Catalytic Spectrophotometry. Results From 0 to${300\mu }\mathrm{g}/\mathrm{L}$iodine content range, the absolute value of correlation coefficient of standard curve of 5 batches exceeded 0.9995, the detection limit was${2.1\mu }\mathrm{g}/\mathrm{L}$; the recovery rate of 9 serum samples with different concentrations was${94.6}\%\sim {108.8}\%;6$batches of 3 serum samples with different concentrations were tested, the RSD was${3.41}\%\sim {4.01}\%$, and the recovery rate was${94.6}\%\sim {108.8}\%$; there was no statistical significance between 20 serum samples and national standard method$\left({t =- {1.660}, P ={0.113}> {0.05}}\right)$. Conclusion Cold digestion method has the advantages of high accuracy and accuracy, simple operation, short time consuming, low demand for temperature control, easy storage and transportation at room temperature.

Engineering rubber products are widely used in construction, transportation, industry, and other fields, and their quality is directly related to the safety and longevity of projects. Therefore, establishing scientific testing standards and quality control techniques is crucial. This paper first introduces the basic characteristics of engineering rubber products and their main application areas, followed by a discussion on the standardized testing process, including physical property testing, chemical composition analysis, and performance evaluation. In terms of quality control techniques, the paper details the control methods during production, the selection and testing of raw materials, and the key stages of finished product inspection. Finally, with reference to current technological advances, the paper looks ahead to the future development trends of testing and quality control technologies for engineering rubber products, particularly focusing on intelligent testing and the use of environmentally friendly materials. This study is of great significance for improving the performance of engineering rubber products, extending their service life, and ensuring project safety, while also providing theoretical and technical support for the formulation of testing standards and quality control specifications in relevant industries.

Objective To explore the regulatory effect of hypericin targeting Nrf2/HO-1 pathway on pancreatic beta cell function in Type 2 diabetes and its potential molecular mechanism. Methods Induced oxidative damage in rat pancreatic beta cell line RIN-m5F by hydrogen peroxide$\left({{\mathrm{H}}_{2}{\mathrm{O}}_{2}}\right)$, pancreatic beta cells were treated with 0,25,50, and 100$\mu$M hyperoside and ML385 (Nrf2 inhibitor). Real time fluorescence quantitative PCR (RT-qPCR) was used to evaluate Nrf2 and HO-1, protein imprinting was used to detect cleaved-Caspase3 and Caspase3, and glucose stimulated insulin secretion assay, 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyltetrazolium bromide thiazole blue, tetrazolium tetrazolium salt (MTT), flow cytometry, and oxidative stress assay kit were used to detect insulin, cell viability, apoptosis, and oxidative stress in rat pancreatic beta cell line RIN-m5F. Results A certain concentration of hyperoside had no cytotoxic effect on RIN-m5F pancreatic beta cells and increased cell survival rate. Hypericin dose dependently reduced apoptosis rate, reactive oxygen species (ROS), and malondialdehyde (MDA) levels, while increasing catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and insulin levels. With the dose-dependent increase of hyperoside, the level of cleaved-Caspase3 significantly decreased, the ratio of cleaved-Caspase3/Caspase3 significantly decreased, and the expression levels of Nrf2 and HO-1 significantly increased. After the addition of Nrf2 inhibitor ML385, the overall expression level decreased, with the same trend as before. Hypericin can activate the levels of antioxidant response elements (ARE) and heat shock protein (HSP90 α), indicating that it can initiate the transcription of a series of antioxidant genes and regulate antioxidant responses. Conclusion Hyperin plays a protective role in Type 2 diabetes islet beta cells by activating Nrf2/HO-1/ARE pathway, and improves the function of islet beta cells.

Objective To study the combined value of Sysmex XN-20 (A1) automated hematology analyzer and blood smear. Methods 280 blood samples from June 2023 to June 2024 were selected as research objects, including 105 abnormal blood samples. Automated hematology analyzer test and blood smear test were performed, the test results were observed and analyzed, and the coincidence rate of automated hematology analyzer, blood smear test alone and combined test was calculated. Results Sysmex XN-20 (A1) automated blood cell analyzer + blood smear detected 105 abnormal blood samples, with a positive test rate of 37.50%(105/280); Sysmex XN-20 (A1) automated The blood cell analyzer detected 100 abnormal blood samples, and the test compliance rate was 35.71%(100/280); the compliance rate of Sysmex XN-20 (A1) automated hematology analyzer + blood smear test was significantly higher, but the two There is no significant difference$\left({P ={0.661},{\chi }^{2}= {0.192}}\right)$. Among the 105 abnormal blood samples, neutrophils had the highest proportion of abnormalities in 27 cases; red blood cells had the second highest proportion of abnormalities in 19 cases; lymphocytes had the third highest proportion of abnormalities in 17 cases. Conclusion The combined application of Sysmex XN-20 (A1) automated hematology analyzer and blood smear can significantly promote the improvement of the coincidence rate of blood specimen test.

Objective To investigate the clinical features, pathogens and drug resistance of elderly patients with traumatic infective endophthalmitis (PTIE). Methods 76 elderly patients (76 eyes) with PTIE treated in our hospital from January 2023 to January 2024 were retrospectively analyzed. Clinical characteristics and pathogenic bacteria of patients were collected and their drug resistance was analyzed. Results The clinical features of elderly PTIE were related to the location of the wound in area I (84.21%), the penetration injury of intraocular foreign body (60.52%) and the presence of metal foreign body (48.68%). The fungal detection rate in patients who were above 75 years old was higher than that in patients${60}\sim {75}$years old$\left({P <{0.05}}\right)$. In 76 elderly patients with PTIE, a total of 56 pathogenic bacteria were isolated, including 45 Gram-positive bacteria, 8 Gram-negative bacteria and 3 kinds of fungi. Among Gram-positive bacteria, the highest resistance of Staphylococcus epidermidis was penicillin (100%) and gentamicin (88.89%), and the highest resistance of Saprophytic staphylococcus was penicillin (88.89%) and erythromycin (88.89%). Among Gram-negative bacteria, the highest resistance was amikacin (100%) and ampicillin (100%) in Pseudomonas aeruginosa, and ampicillin (100%) in Klebsiella pneumoniae. Conclusion The distribution of pathogenic bacteria in elderly PTIE patients was affected by the location of the injured mouth, the type of trauma, the type of foreign body in the eye and age factors, among which Gram-positive bacteria was the main infection type, Staphylococcus epidermidis was the most common. Staphylococcus epidermidis has a high resistance to penicillin, in contrast to ampicillin and imipenem are relatively low resistance.

Objective To compare the results of high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) and chemiluminescence immunoassay (CLIA) in the determination of serum estradiol (E2) level. Methods From January 2023 to January 2024, 100 patients who were treated in outpatient department and hospitalized in the hospital were selected as the research subjects. Fresh blood samples were collected from all study subjects on the same day, and serum E2 levels were measured by LC-MS/MS and CLIA respectively. Results Serum E2 level measured by CLIA was significantly higher than that of LC-MS/MS ($P <{0.05}$). Pearson correlation test results showed that LC-MS/MS was significantly correlated with CLIA in measuring serum E2 level$\left({r ={0.866}, P <{0.05}}\right)$. There was a linear correlation between serum E2 level measured by LC-MS/MS and CLIA, and the linear regression equation was manifested as$Y ={1.161X}-{6.574}$ $\left({r ={0.973}, n={100}}\right)$. Bland-Altman analysis revealed that the average difference between LC-MS/MS and CLIA in measuring serum E2 level was${0.078}\mathrm{{nmol}}/\mathrm{L}\left({{95}\%\mathrm{{CI}}:{0.069}\sim {0.086}\mathrm{{nmol}}/\mathrm{L}}\right)$, and the consistency limit range was (-0.011,0.143). There were 5%(5/100) points outside the 95% consistency limit range, and within the 95% consistency limit range, and the maximum difference between LC-MS/MS and CLIA was${0.156}\mathrm{{nmol}}/\mathrm{L}$. The average value of serum E2 level measured by LC-MS/MS and CLIA was${0.152}\mathrm{{nmol}}/\mathrm{L}$, suggesting that the consistency of the two methods was poor. Conclusion Serum E2 level determined by CLIA is higher than that determined by LC-MS/MS. There is a certain correlation between the two methods for the determination of serum E2 level, but the consistency is not good. The determination of serum E2 level by CLIA is more suitable for rapid screening. When accurate clinical diagnosis is needed, it is necessary to apply LC-MS/MS to measure serum E2 level.

This article aims to investigate the common technical issues regarding the non-excavation detection of the anti-corrosion coating condition of long-distance pipelines, and propose solutions and technical measures. Among the external corrosion detection methods for the regular inspection of long-distance pipelines, the non-excavation detection of the anti-corrosion coating condition detection is a major aspect. The PCM (Pipeline Current Mapper) testing technology is used. The quality of its detection determines the detection result of damaged areas in the anti-corrosion coating and the safety of long-distance pipeline operation. On the basis of analyzing the significance of PCM detection for long-distance pipelines, this article explores the prevalent problems encountered in PCM detection of long-distance pipelines. In combination with years of practical operation experience and relevant national regulations, targeted solutions and treatment approaches are proposed.

Material surface performance testing technology plays an important role in the research and application of chemistry, especially in university chemistry experimental teaching. These technologies provide students with opportunities to deeply understand the surface characteristics, structure, and chemical behavior of materials. This article focuses on the commonly used surface performance testing techniques for materials, such as scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), contact angle measurement, surface plasmon resonance (SPR), and Raman spectroscopy. It analyzes their practical applications in university chemistry experiments and their impact on improving students' experimental abilities and scientific research literacy. By introducing these advanced detection techniques in experimental teaching, not only can students enhance their understanding of the microstructure and chemical reaction mechanism of material surfaces, but they can also help them master the commonly used technical means in modern scientific research, laying a solid foundation for future scientific research work.

Against the backdrop of rapid global urbanization and booming construction industry, the demand for various materials in construction projects is showing explosive growth. Whether it is the concrete used to build sturdy foundations, the steel bars that bear the structural strength, or the sand, gravel, and cement used to construct foundations, their roles in the construction blueprint are crucial. They must meet the strictest quality specifications and performance indicators to ensure the stability and durability of the project. However, the fierce competition in the market, the complexity of the supply chain, and the lack of regulatory oversight have made the quality of the building materials market quite delicate. This may not only breed engineering defects and bury safety hazards, but also cause unnecessary loss of valuable resources. This article first analyzes the important role of building material testing in construction engineering, discusses the content of building material testing and the hazards caused by inadequate testing work, and focuses on exploring optimization strategies for building material testing in construction engineering for reference.