Objective To analyze the quality control results of detecting influenza virus nucleic acid by fluorescence real-time quantitative PCR. Methods From May 2021 to November 2022, 120 samples of influenza virus infection were studied. The RNA of influenza virus in these samples was extracted by nucleic acid and detected by PCR real-time fluorescence quantitative technique to evaluate its effect. Results 44 cases of influenza B virus, 40 cases of influenza A 1 virus and 36 cases of influenza A 3 virus were detected by this technique. All the test results meet the quality control standards of molecular biology operation, and the qualified rate reaches 100.0%. Conclusion Real-time nucleic acid PCR technology is widely used in clinical influenza virus detection. PCR real-time fluorescence quantitative detection method is accurate and rapid in the detection of influenza virus nucleic acid, which is suitable as the first choice to deal with public health emergencies.

Grain and oil safety is related to the foundation of the safety of the entire food chain, and the testing of grain and oil institutions provides important technical support for government supervision. Quality control lies at the core of institutional testing, which not only ensures the accuracy and reliability of testing data, but also provides basic guarantee for establishing a scientific and sound testing system in testing institutions. This paper summarizes the main methods, monitoring links, and analyzes existing problems and challenges of quality control in grain and oil testing institutions. It proposes four suggestions for improving the further effectiveness of quality controlto provide reference for peers.

Objective To investigate the distribution of hepatitis Bvirus (HBV) genotypes and drug resistance mutation sites in Kaifengarea. Methods A total of 200 hepatitis B surface antigen (HBsAg)positive patients were selected from Kaifeng area from January 2018 toDecember 2020. The genotype of the patients was detected by genesequencing technology, and the test results were statistically analyzed.Nucleoside analogues (NAs) resistance gene loci in serum HBV-DNA of 200untreated patients were analyzed. Results Among 200 HBsAg positivepatients in Kaifeng area, 179 cases were genotype C, accounting for ${97.8}\%,4$ cases were genotype B,accounting for ${2.2}\%$ , and no B+Cor other types were detected. Among the 200 patients, 26 were detectedNAs gene drug resistance mutation sites, and the detection rate of drugresistance mutation sites in patients with gene $\mathrm{C}$ was higher than that inpatients with gene B. Conclusion Hepatitis B surface antigen positivepatients in Kaifeng area of HBV genotype mainly $\mathrm{C}$ type, B type is less, no othergenotype was detected. The detection rate of NAs resistance sites ingenotype C was higher than that in genotype B. A total of 9 differentcombinations of NAs drug-resistant mutation sites were detected, mainlyunit point mutation.

Objective To evaluate the analytical performance of serumamyloid A protein and C-reactive protein detection reagents based onmicrofluidic technology, so as to determine whether they can meetclinical requirements. Methods The precision, accuracy, linear range,interference test and other technical indicators of the detectionreagent were determined according to the CLSI EP scheme. Results Theintra-batch coefficients of variation of high and low concentrationsamples were 0.54% and 1.79%, and the inter-batch coefficients ofvariation were 0% and 1.20%, respectively, for the serum amyloid Adetection reagent; the intra-batch coefficients of variation of high andlow concentration samples were 0.30% and 2.28%, and the inter-batchcoefficients of variation were 0.17% and 0%, respectively, for theC-reactive protein detection reagent; the experimental results of themethodological comparison of the two methods were $\mathrm{Y}=$ -0.0609+0.9871X, ${\mathrm{R}}^{2}= {0.9975}$ and $\mathrm{Y}= {0.3354}+{0.9857}\mathrm{X},{\mathrm{R}}^{2}= {0.9990}$ , respectively,and the allowable errors were greater than the upper limit of theexpected bias confidence interval, and the biases were acceptable; thelinear range of the serum amyloid A detection reagent was $3 \sim {100.0}\mathrm{{mg}}/\mathrm{L}$ ,and the linear range of the C-reactive protein detection reagent was $3\sim {150.0}\mathrm{{mg}}/\mathrm{L}$ . Conclusion The analytical performance of serum amyloid A and C-reactive protein detection reagents based on microfluidic technology can meet the needs of clinical laboratories.

The treatment and detection of nickel-containing electroplating wastewater is an important topic in the field of environmental protection. This paper analyzes the generation and composition of nickel-containing wastewater in the electroplating process, and discusses the impact of nickel-containing wastewater on the environment and human health. In order to improve the detection accuracy and efficiency, this paper optimizes the existing detection methods, including the improvement of pre-treatment technology, the optimization of detection equipment and methods, and the application of automated and intelligent detection systems. The experimental study verified the effectiveness of the optimized detection method in improving sensitivity, shortening detection time, and reducing detection costs, providing technical support for the effective management and treatment of electroplating wastewater.

This paper aims to discuss the key points of quality control of on-site testing in mine safety inspection laboratory, including the quality and skills of analysts, the management and maintenance of instruments and equipment, the specification of sample management, and the condition control of environmental facilities. Through the implementation of a strict quality control system and the application of highly automated inspection technology, the accuracy and trust of inspection data can be significantly improved. Formulate specific requirements and implementation strategies for laboratory personnel training, instrument maintenance system, sample management process and environmental condition control, so as to comprehensively improve the quality of on-site testing work.

Objective Determination of silicon dioxide in fluorite byalkali fusion-molybdenum blue spectrophotometry. Methods The method ismelted with potassium hydroxide, extracted with water and acidified withsulfuric acid, then, in a weak acidic solution, silicic acid andammonium molybdate form soluble yellow silicomolybdic heteropoly acids,to improve acidity, reduce silicomolybdate yellow to silicomolybdateblue by reducing agent, the absorbance was measured at ${700}\mathrm{\;{nm}}$ and the silicondioxide content was measured. A large number of fluoride interference,the addition of aluminum salt to eliminate. Results Determination ofsilicon dioxide in fluorite by alkali fusion-molybdenum bluespectrophotometry, the silicon dioxide has good calibration curvelinearity, the linear regression equation is $\mathrm{Y}= {0.34733}\mathrm{p}+{0.00228}$ , the correlation coefficient was 0.999; the precisionof the method is less than 1.5%, the accuracy was measured with thenational reference materials, and the relative standard deviation (RSD)was less than 2%. Conclusion It was found that the precision andaccuracy of this method for the determination of silicon dioxide influorite samples were satisfactory and met the requirements of relevantstandards.

As an inevitable product in coal mine production, dust has a wide range of sources. These dust not only have complex physical and chemical properties, but their concentration and distribution characteristics are also affected by various factors. This article aims to comprehensively and systematically study the characteristics and hazards of coal mine underground dust through fixed-point sampling and detection analysis methods. By selecting and arranging sampling points, preparing and calibrating sampling instruments, and performing specific operations during the sampling process, the accuracy of data is ensured. At the same time, based on the detection results, the concentration, particle size distribution, chemical composition, and explosiveness of dust are revealed, in order to provide effective basis for the development of targeted dust prevention measures.

Objective To evaluate and analyze the value of halophilicbacteria culture in the detection of Vibrio parahaemolyticus in seafoodin Dalian area. Methods 46 samples of Marine products were collectedfrom Dalian from July 2023 to October 2023, and halophilic bacteria werecultured and isolated. The salt-tolerance test was carried out toidentify and analyze the virulence of Vibrio parahaemolyticus, and thecharacteristics and detection of Vibrio parahaemolyticus in Marineproducts were analyzed. Results A total of 45 halophilic and salttolerant strains were screened, with a yield of 44.44% at 20% salinity,55.56% at 15% salinity, and 11.11% at 50°C cultivation environment. Theisolated strains continued to grow at a concentration of ${25}\%\mathrm{{NaCl}}$ , and the bestgrowth was observed at a concentration of ${10}\%\mathrm{{NaCl}}$ . Seventeenmoderately halophilic bacteria were detected, with a total of 28 salttolerant strains. Conclusion The detection rate of Vibrioparahaemolyticus in Marine products in Dalian area can be improved byusing halophilic bacteria culture detection. The results of virulenceidentification show that Vibrio parahaemolyticus has strong virulenceand potential risk of food poisoning.

With the improvement of people's living standards, food safety issues have become the focus of attention. Food microorganisms as an important indicator of food hygiene, which has an important role in ensuring food safety. To a large extent, determine the accuracy of the test results. This article conducts research and discussion of process control from the entire link of the medium procurement, acceptance, storage, preparation, use, and abandonment of the medium, which explains the measures to improve the quality of the medium. It is expected to provide some references for the effective development of food microorganisms.