Objective To evaluate and analyze the value of halophilicbacteria culture in the detection of Vibrio parahaemolyticus in seafoodin Dalian area. Methods 46 samples of Marine products were collectedfrom Dalian from July 2023 to October 2023, and halophilic bacteria werecultured and isolated. The salt-tolerance test was carried out toidentify and analyze the virulence of Vibrio parahaemolyticus, and thecharacteristics and detection of Vibrio parahaemolyticus in Marineproducts were analyzed. Results A total of 45 halophilic and salttolerant strains were screened, with a yield of 44.44% at 20% salinity,55.56% at 15% salinity, and 11.11% at 50°C cultivation environment. Theisolated strains continued to grow at a concentration of ${25}\%\mathrm{{NaCl}}$ , and the bestgrowth was observed at a concentration of ${10}\%\mathrm{{NaCl}}$ . Seventeenmoderately halophilic bacteria were detected, with a total of 28 salttolerant strains. Conclusion The detection rate of Vibrioparahaemolyticus in Marine products in Dalian area can be improved byusing halophilic bacteria culture detection. The results of virulenceidentification show that Vibrio parahaemolyticus has strong virulenceand potential risk of food poisoning.

halophilic bacterial culture  /  seafood  /  Vibrio parahaemolyticus  /  food safety