Objective To analyze the quality control results of detecting influenza virus nucleic acid by fluorescence real-time quantitative PCR. Methods From May 2021 to November 2022, 120 samples of influenza virus infection were studied. The RNA of influenza virus in these samples was extracted by nucleic acid and detected by PCR real-time fluorescence quantitative technique to evaluate its effect. Results 44 cases of influenza B virus, 40 cases of influenza A 1 virus and 36 cases of influenza A 3 virus were detected by this technique. All the test results meet the quality control standards of molecular biology operation, and the qualified rate reaches 100.0%. Conclusion Real-time nucleic acid PCR technology is widely used in clinical influenza virus detection. PCR real-time fluorescence quantitative detection method is accurate and rapid in the detection of influenza virus nucleic acid, which is suitable as the first choice to deal with public health emergencies.

real-time fluorescence quantification  /  PCR detection  /  influenza virus nucleic acid  /  quality control results