Objective To clarify the detection status and antimicrobial sensitivity of clinically isolated Pseudomonas aeruginosa, assist clinical doctors in using antibiotics based on evidence, delay the rate of bacterial resistance, and intervene purposefully in hospital acquired infections. Methods Pseudomonas aeruginosa cultured, isolated, and identified from clinical samples submitted by our company from January 1, 2023 to December 31, 2023 were collected. According to the latest standard CLSI-M100-ED33, the bacteria were identified using a time-of-flight mass spectrometer, and a fully automated identification and drug susceptibility analysis system was used for drug susceptibility testing. Results Pseudomonas aeruginosa was mainly isolated from elderly men over 60 years old. Sputum was the main source of infection specimens of Pseudomonas aeruginosa. The infected patients were mainly distributed in respiratory and internal medicine departments. The isolated Pseudomonas aeruginosa had high resistance to antibiotics such as amtraxam and ciprofloxacin, and high sensitivity to amikacin and tobramycin, and the overall antibiotic resistance showed a downward trend. Conclusion Strict detection of bacterial resistance and drug resistance genes should be carried out in clinical medicine, so that antibiotics can be used more rationally.

Objective Establish a ultra high performance liquidchromatography (UPLC) method for the simultaneous determination of sevenginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd in ginseng medicinalmaterials. Methods Using CAPCELL PAK ${\mathrm{C}}_{18}\mathrm{{MG}}\left({{2.0}\mathrm{\;{mm}}\times {150}\mathrm{\;{mm}},{2.0\mu}\mathrm{m}}\right)$ Chromatographic column with gradientelution of acetonitrile-0.05% phosphoric acid solution as mobile phase,flow rate of ${0.38}\mathrm{\;{mL}}\cdot {\mathrm{{min}}}^{-1}$ ,detection wavelength of ${203}\mathrm{\;{nm}}$ , column temperatureof ${30}^{\circ }\mathrm{C}$ , andinjection volume of ${1\mu}\mathrm{L}$ . Results All 7 ginsenosides can be completelyseparated and have a good linear relationship $\left({{r}^{2}> {0.999}}\right)$ withina certain linear concentration range, with an average recovery rate of ${97.22}\%\sim {104.56}\%$ ; Therelative standard deviation (RSD) is ${0.4}\%\sim {1.8}\%\left({n = 9}\right)$ ,and the precision RSD is ${0.57}\%\sim {1.42}\%$ . Conclusion Thismethod has strong specificity, excellent precision, good accuracy, andmore efficient detection process, and can be used for the determinationof seven ginsenosides in ginseng medicinal materials.

Objective To establish a rapid, accurate and sensitive gaschromatography-mass spectrometry (GC-MS) method for the detection ofdichloroacetic acid (DCA) and trichloroacetic acid (TCA) in drinkingwater samples, and to evaluate the performance and practical applicationof the method. Methods The samples were pretreated by liquid-liquidextraction derivatization technology and then analyzed by GC-MS. ${50\mu }\mathrm{L}$ and ${100\mu }\mathrm{L}$ of DCA and TCAstandards were prepared into mother solutions with concentrations of ${5\mu }\mathrm{g}/\mathrm{{mL}}$ and ${10\mu }\mathrm{g}/\mathrm{{mL}}$ ,respectively, and then diluted with methanol to form a series ofstandard solutions with concentration gradients for the establishment ofstandard curves. 1,2-Dibromopropane was used as the internal standardsubstance to prepare an internal standard extract of ${166.1\mu }\mathrm{g}/\mathrm{L}$ tocorrect the error in the sample analysis process. The spike recoveryexperiment was carried out on ultrapure water to evaluate the accuracyand precision of the method, and the detection limit of the method wasdetermined by the signal-to-noise ratio (S/N) method. Finally, 103drinking water samples were tested to analyze the contents of DCA andTCA. Results The linear ranges of the standard curves were $1 \sim {20}\mathrm{{\mug}}/\mathrm{{mL}}$ for DCA and $1\sim {40}\mathrm{{\mu g}}/\mathrm{{mL}}$ for TCA, withcorrelation coefficients $\left({r}^{2}\right)$ greater than 0.99,recoveries of 99.4%~114.3%, RSDs less than 3.541%, and detection limitsof ${0.1\mu }\mathrm{g}/\mathrm{L}$ and ${0.2\mu }\mathrm{g}/\mathrm{L}$ for DCA and TCA, respectively. The contents of DCA and TCA in 103drinking water samples were lower than the national standard limits,with average concentrations of ${0.25\mu}\mathrm{g}/\mathrm{L}$ and ${0.48\mu}\mathrm{g}/\mathrm{L}$ , respectively, and the data werestatistically significant $\left({P<{0.05}}\right)$ . Conclusion The GC-MS method has good linearity, accuracy, precision and sensitivity, and can meet the needs of drinking water safety monitoring. The method was applied to 103 drinking water samples, and the results showed that the drinking water quality met the national standards and was not seriously contaminated by DCA and TCA.

Objective To compare the diagnostic value and healtheconomic value of rapid detection of mycobacterium tuberculosis andrifampicin resistance (XpertMTB/RIF), mycobacterium nucleic acidPCR-fluorescent probe detection (MTB-NTM-PCR) and RNA isothermalamplification real-time fluorescence detection (SAT-TB) in detectingbronchial alveolar lavage fluid (BALF) in smear-negative pulmonarytuberculosis Be worth. Methods A retrospective analysis was conducted on100 patients with clinically diagnosed pulmonary tuberculosis butnegative sputum smears admitted to Guiyang Public Health TreatmentCenter from January 2019 to July 2022. All patients had negative sputumsmears for three consecutive times and underwent fiberoptic bronchoscopyin the fiberoptic bronchoscopy room of our hospital. BALF specimens weresimultaneously tested by smear acid-fast staining, GeneXpert MTB/RIF,MTB-PCR and SAT-TB detection methods. Taking the final clinicaldiagnosis of pulmonary tuberculosis as the gold standard, the diagnosticefficiency of the three methods and cost-effectiveness analysis werecalculated. Results The positive rates of XpertMTB/RIF, MTB-PCR andSAT-TB in 100 BALF samples were 53.00%, 43.00% and 35.00%, respectively.Taking clinical diagnosis as the gold standard, the positive rates ofXpertMTB/RIF $\left({{\chi }^{2}= {21.459}, P<{0.001}}\right)$ , MTB-PCR $\left({{\chi }^{2}= {17.695}, P<{0.001}}\right)$ , SAT-TB $\left({{\chi }^{2}= {12.631}, P<{0.001}}\right)$ and clinical diagnosis were statisticallysignificant. The sensitivity of XpertMTB/RIF, MTB-PCR, SAT-TB,XpertMTB/RIF+MTB-PCR, XpertMTB/RIF+SAT-TB, MTB-PCR+SAT-TB and the threecombined detections were 64.20%, 53.09%, 43.21% and 67, respectively ${90}\%,{66.67}\%,{58.02}\%,{69.14}\%$ ; Thespecificity was ${94.74}\%,{100.00}\%,{100.00}\%,{94.74}\%,{94.74}\%,{100.00}\%$ ,94.74%, respectively. ROC analysis showed that The AUC of XpertMTB/RIF,MTB-PCR, SAT-TB, XpertMTB/RIF+MTB-PCR, XpertMTB/RIF+SAT-TB,MTB-PCR+SAT-TB and the combined detection were 0.795, 0.765, 0.716 and0.813, respectively 0.807, 0.790, 0.819. Conclusion In the rapiddetection of negative tuberculosis, it is not recommended to select theabove three tests at the beginning of diagnosis and treatment. In termsof cost effectiveness, MTB-PCR can be used as the preferred diagnosticmethod for smear-negative pulmonary tuberculosis.

With the rapid development of China's economic market, the safety evaluation of mechanical and electrical products has become a key link for enterprises to enhance their competitiveness. Through the application of systematic functional risk assessment, the standardization and scientific nature of safety evaluation of mechanical and electrical products can be improved. This paper mainly analyzes the difference between mechanical and electrical product safety evaluation and general evaluation and the specific application of functional hazard assessment in mechanical and electrical product safety evaluation. The results show that functional hazard assessment can comprehensively and scientifically evaluate the safety performance of mechanical and electrical products, and provide strong support for the selection of products with higher cost performance. It also provides useful reference and guidance for practical application, and further promotes the development of safety evaluation of electromechanical products in our country.

Objective Gray mold is an important disease in thestrawberry production process, which seriously affects its yield andquality. This article aims to study the resistance of gray moldpathogens in different strawberry production areas in Zhejiang Provinceto fludioxonil, in order to clarify the current use status and futuremedication strategies of this fungicide. Methods This article uses themycelial growth rate method to conduct indoor toxicity testing on 184strains isolated from strawberry gray mold disease samples in 7 regionsof Zhejiang Province, in order to determine their sensitivity tofludioxonitril and count the frequency of occurrence of resistant andsensitive strains. Results The results showed that the strawberry graymold strain in Quzhou area had the highest sensitivity to fludioxonil,with an average ${\mathrm{{EC}}}_{50}$ value of ${0.014\mu }\mathrm{g}/\mathrm{{mL}}$ ,while the strawberry gray mold strain in Huzhou area had the lowestsensitivity to fludioxonil, with an average ${\mathrm{{EC}}}_{50}$ value of ${0.032\mu }\mathrm{g}/\mathrm{{mL}}$ and asensitivity baseline of ${0.018\mu}\mathrm{g}/\mathrm{{mL}}$ and the sensitive baseline value forthe whole province is ${0.018\mu}\mathrm{g}/\mathrm{{mL}}$ . Conclusion At present, a few lowresistant strains have appeared in the Zhejiang region, indicating thatthe strawberry gray mold fungus has developed certain resistance tofludioxonitril. The sensitivity baseline value has increased compared toprevious research results, but the overall level of drug resistance isstill controllable.

Objective To establish an extraction process and contentdetermination method for quercetin in Gentiana macrophylla, and optimizethe processing technology of Gentiana macrophylla. Methods This studyused ultrasound assisted extraction to extract quercetin from Gentianamacrophylla, and determined the content of quercetin in four processedGentiana macrophylla decoction pieces by HPLC-ELSD. Results The optimalprocess conditions for ultrasonic extraction of quercetin from Gentianamacrophylla are: ethanol volume fraction of 75%, ultrasonic time of 45minutes, solid-liquid ratio of 1 : 10, and ultrasonic temperature of ${30}^{\circ }\mathrm{C}$ . Thehighest yield of quercetin is ${3.40}\mathrm{{mg}}/\mathrm{g}$ . Conclusion The established extraction process and content determinationmethod for quercetin galls have good repeatability, stability, andreliability, which can provide reference for optimizing the processingtechnology of Gentiana macrophylla decoction pieces.

Objective To establish a method for determining thecontents of paeoniflorin, cinnamaldehyde and paeonol in Guizhi JiliuPills. Methods The RP-HPLC wavelength switching method was used, theanalysis was achieved on an ES Epic ${\mathrm{C}}_{18}\left({{4.6}\mathrm{\;{mm}}\times {250}\mathrm{\;{mm}},{5\mu}\mathrm{m}}\right)$ column, the mobile phase was acetonitrile-0.1% aqueous solution of phosphoric acid gradient elution, the flowrate was ${1.0}\mathrm{\;{mL}}/\mathrm{{min}}$ , thecolumn temperature was ${25}^{\circ }\mathrm{C}$ and thedetection wavelength were ${230}\mathrm{\;{nm}}$ $\left({0\sim {25}\mathrm{\;{min}}}\right),{290}\mathrm{\;{nm}}\left({{25}\sim {41}\mathrm{\;{min}}}\right)$ and ${274}\mathrm{\;{nm}}\left({{41}\sim {51}\mathrm{\;{min}}}\right)$ . Results The linear ranges of paeoniflorin, cinnamaldehyde and paeonolwere 3.6169~90.4232 μg/mL ( $r={0.9993}$ ),1.8388~45.9695 μg/mL ( $r={0.9999}$ ),2.0240~50.5994 μg/mL ( $r={0.9999}$ ). The average sample recovery rates were95.4%(RSD=1.2%), 93.0%(RSD=0.7%) and 94.2%(RSD=1.2%) respectively. Conclusion This method is accurate, sensitive, and has strong specificity. It can be used for the quality control of Guizhi Jiliu Pills.

Objective To explore the determination of trace glutathioneby resonance scattering spectral quenching method and improve thesensitivity of the method. Methods In the pH 3.0 phosphoric acid-boricacid-acetic acid buffer solution (BR buffer), $\mathrm{{Fe}}\left(\mathrm{{III}}\right)$ reacts with ${\mathrm{K}}_{4}\left\lbrack {\mathrm{{Fe}}{\left(\mathrm{{CN}}\right)}_{6}}\right\rbrack$ to form Prussian blue ion associates, and the system exhibits a strongresonance scattering effect at ${337}\mathrm{\;{nm}}$ . When glutathione isadded, the sulfhydryl group in glutathione molecules can reduce Fe(III)to Fe(II), causing quenching of the system and weakening the resonancescattering quenching effect. Results The linear regression equation forglutathione within the concentration range of 0.010 to ${2.0\mu }\mathrm{g}/\mathrm{{mL}}$ is ${\Delta l}= {299.77\rho }+{774.52}$ , with an ${r}^{2}$ of 0.9929 and a detection limit of ${0.0024\mu }\mathrm{g}/\mathrm{{mL}}$ . Conclusion This method is simple and highly sensitive, and satisfactoryresults are obtained when used for the determination of glutathione indrugs.

University laboratory as an important base of scientific research and teaching, safety management is very important. Effective laboratory management plays an important role in ensuring the smooth operation of practical teaching and scientific research and the safety of teachers and students. This study focuses on the current situation and challenges of the safety management of specialized biological laboratories related to agriculture, focusing on four core elements of laboratory management system, reagent management, equipment management and waste liquid treatment. Through the construction of scientific management system, explore the safety management system of biological laboratories related to agriculture, aiming to improve the safety management ability of scientific research laboratories in local universities under the background of new agricultural science.