Objective To study the preparation technology anddetermination of compound tetrahydropalmatine pain relief gel. MethodsOrthogonal test was performed to determine the prescription ratio of thegel. RP-HPLC method was adopted. The determination was performed onEclipce XDB-C18 ( ${200}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) column with mobile phase consisted ofmethanol-0.6% glacial acetic acid (pH 6.0) 30 : 70 . The detectionwavelength was ${280}\mathrm{\;{nm}}$ , the flow rate was $1\mathrm{\;{mL}}/\mathrm{{min}}$ .Results With $1\mathrm{\;g}$ carbomer ${940},{20}\mathrm{\;g}$ propyleneglycol, $8\mathrm{\;g}$ glycerol, ${0.5}\mathrm{\;g}$ triethanolamine as thegel matrix, the linear ranges of tetrahydropalmatine and imperatorinwere ${16.1}\sim {96.6}\mathrm{{\mug}}/\mathrm{{mL}}\left({r ={0.9999}}\right)$ and ${10.4}\sim {62.4}\mathrm{{\mug}}/\mathrm{{mL}}$ $\left({r={0.9999}}\right)$ , respectively. The average recoveries were99.13%(RSD was 0.79%) and 99.26%(RSD was 0.82%), respectively.

Lactic acid and its derivatives are important flavor substances in Nongxiang Baijiu which play a vital role in the aroma and taste of the wine. In order to screen lactic acid bacteria, this study tooke a Nongxiang Baijiu fermented grains as the research object. The isolation conditions of lactic acid bacteria were optimized, the effects of different DNA extraction methods were compared, and the species of lactic acid bacteria were identified by enzyme digestion and DNA sequencing. The results showed that 95 strains of lactic acid bacteria were preliminarily screened in MRS broth medium under anaerobic culture at 37°C for 24 h. The optimal method for DNA extraction of lactic acid bacteria was determined to be boiling method, and the strains were identified as Lactobacillus panis, which had the effects of producing lactic acid and n-propanol. This study has important reference value for the production and quality improvement of Nongxiang Baijiu.

Objective To add data output and processing functions to ordinary models of UV visible spectrophotometers. Methods Connect the spectrophotometer to an Android system phone using a serial port to USB cable. The phone uses serial software to receive and process the measurement data sent by the spectrophotometer. Results The connection between the spectrophotometer and the mobile phone was successfully achieved. The mobile phone can smoothly receive and store the output data of the spectrophotometer, and perform graphical processing, synchronously drawing the wavelength absorbance scanning spectrum of the sample. The obtained absorption spectrum is basically consistent with the commercial instrument. Conclusion A simple modification scheme for the output and processing of ordinary spectrophotometer data based on Android system smartphones has been proposed. The modification method is low-cost, easy to operate, and ordinary UV visible spectrophotometers with serial interfaces can be upgraded and modified.

The purpose of this paper is to discuss the application of Liquid Chromatography-Mass Spectrometry (LC-MS) in drug quality analysis in order to improve the accuracy and efficiency of drug detection. The basic principle, equipment and application of LC-MS in drug quality analysis are reviewed in this paper. LC-MS technology has shown excellent performance in the qualitative, quantitative, fingerprint and pharmacokinetics of drugs, providing an efficient and accurate tool for drug quality analysis, and has a broad application prospect.

Food contaminants pose a serious challenge to public health and the food industry, and there is an urgent need to develop more advanced detection techniques and updated prevention strategies. Although the traditional culture technology has played an important role in the detection of food contaminants, there are obvious limitations in the detection speed, sensitivity and specificity. With the increasing expansion of global food trade, the growing production scale of food enterprises, and the increasing attention of the public to food safety, food safety has become a hot topic around the world. Food contaminants are mainly divided into chemical and biological categories, including but not limited to pesticide and veterinary drug residues, heavy metal ions, abused food additives, foodborne pathogens and mycotoxins. In recent years, with the rapid development of cutting-edge technologies such as molecular biology technology, biosensors, nanotechnology and advanced imaging technology, these innovative methods are gradually overcoming the limitations of traditional detection methods. This paper discussed the principles, advantages and limitations of these innovative detection technologies in depth, and reviewed the latest research progress in the field of food detection. By comparing different detection methods, this paper aims to provide guidance for selecting suitable detection technology to achieve rapid and accurate detection and analysis of food contaminants.

Against the backdrop of increasingly collaborative global scientific research, regional joint laboratories have gradually gained attention as important platforms for improving research efficiency and transforming achievements. As an effective platform for integrating regional research resources and promoting deep integration of industry, academia, and research, research consortia can play a key role in enhancing regional innovation capabilities of regional joint laboratories and accelerating the transformation of scientific and technological achievements. This article aims to explore the construction and open sharing strategy of regional joint laboratories based on research consortia, analyze the challenges and opportunities faced in the current construction and operation of regional joint laboratories, and propose corresponding countermeasures and suggestions.

Objective To explore the pathogenic microbial detection results and drug susceptibility results of children with bacterial diarrhea after the collection of stool samples. Methods From April 2020 to June 2023,170 children with bacterial diarrhea participated in the study. The family members were informed about the study, and assisted the children to complete the collection of stool specimens. All the specimens were sent to the laboratory for pathogen culture, counted the distribution of pathogens, and analyzed the drug resistance of pathogens. Results 170 stool samples isolated 122 pathogenic strains, The detection rate of gram-negative bacteria was 76.23%, mainly Salmonella (27.87%), diarrheagenic Escherichia coli (21.31%), Shigella (21.31%); The detection rate of gram-positive bacteria was 23.77%, mainly Staphylococcus aureus (22.95%); Most resistance to tetracycline (41.18%), ciprofloxacin (38.24%), ampicillin (35.29%), The greatest resistance to ciprofloxacin (46.15%), tetracycline (38.46%), of ciprofloxacin (53.85%), tetracycline (46.15%), Staphylococcus aureus had to clindamycin (53.57%) and penicillin (53.57%). Conclusion Salmonella, Escherichia coli, Shigella and Staphylococcus aureus are the main causes of bacterial diarrhea in children. The drug resistance and the choice of sensitive drugs are conducive to the early recovery of children.

As important bases for experimental teaching and medical research, the biosecurity of laboratories in medical universities cannot be ignored. In order to accurately grasp the research progress of biosecurity management in medical university laboratories in China, co-word cluster analysis was conducted on the 473 literatures related to biosecurity in medical laboratories published in the CNKI database from 2004 to 2023 using CiteSpace software. The research results showed that biosecurity, laboratory safety management, safety education, and medical testing are the four current research hotspots in this field, while medical research laboratories and safety education are potential directions for future research development.

Objective To investigate the accuracy of chemiluminescenceimmunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) in thedetection of serum markers in patients with HBV infection, and toanalyze the quantitative correlation between serum markers and hepatitisB virus deoxyribonucleic acid (HBV-DNA). Methods A total of 86 patientswith hepatitis B admitted to our hospital from January 2023 to January2024 were selected as subjects. Two immunological detection methods wereused to detect HBV serum markers, and the accuracy of the two detectionmethods was analyzed. Real-time fluorescent polymerase chain reaction(qRT-PCR) was used for quantitative analysis of HBV-DNA load. McNemar’stest was used to analyze the quantitative correlation between serummarkers and HBV-DNA. Results The positive rates of HBsAg, HBsAb andHBeAg were moderate, but the positive rates of HBeAb and HBcAb werestrong. With ELISA as the gold standard, the sensitivity and specificityof CLIA assay were 97.30% and 75.00%, while with CLIA as the goldstandard, the sensitivity and specificity of ELISA assay were 96.00% and81.82%. Among the 6 serum models, the positive rate of HBV-DNA was ${95.65}\%$ , and the mean quantity ofHBV-DNA was ${1.12}\times {10}^{8}$ copies/mL, which was significantly higher than that of the other 5models. The positive rate of HBV-DNA in Xiaosanyang was also relativelyhigh (65.63%). Conclusion The two methods have strong consistency indetecting serum markers of HBV infection, but the

Objective To optimize the ultrasonic extraction technologyof flavonoids, polyphenols and saponins from Mirabilis himalaica, and toevaluate their antioxidant activities. Methods Based on the singlefactor tests of ethanol concentration, liquid-solid ratio, extractiontemperature and extraction time, Box-Behnken response surface method wasused to optimize the extraction process of flavonoids, polyphenols andsaponins. The antioxidant activity of DPPH, ABTS, superoxide anion andhydroxyl radical was investigated in vitro. Results The optimizedultrasonic extraction technology was ${60}\%$ ethanol concentration, ${30}\mathrm{\;{mL}}/\mathrm{g}$ liquid-solid ratio, 50℃ extractiontemperature and 55 min extraction time. The extracts had strongscavenging effect on free radicals. Conclusion It is proved that theoptimized ultrasonic extraction technology of flavonoids, polyphenolsand saponins from Mirabilis himalaica is reasonable, and the extractshave antioxidant activity in vitro.