Objective To optimize the ultrasonic extraction technologyof flavonoids, polyphenols and saponins from Mirabilis himalaica, and toevaluate their antioxidant activities. Methods Based on the singlefactor tests of ethanol concentration, liquid-solid ratio, extractiontemperature and extraction time, Box-Behnken response surface method wasused to optimize the extraction process of flavonoids, polyphenols andsaponins. The antioxidant activity of DPPH, ABTS, superoxide anion andhydroxyl radical was investigated in vitro. Results The optimizedultrasonic extraction technology was ${60}\%$ ethanol concentration, ${30}\mathrm{\;{mL}}/\mathrm{g}$ liquid-solid ratio, 50℃ extractiontemperature and 55 min extraction time. The extracts had strongscavenging effect on free radicals. Conclusion It is proved that theoptimized ultrasonic extraction technology of flavonoids, polyphenolsand saponins from Mirabilis himalaica is reasonable, and the extractshave antioxidant activity in vitro.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |