To compare the antibacterial (against Escherichia coli/Salmonella), antiviral (against HSV-1/2), and NF-κB inhibitory activity of sea buckthorn leaf extracts (SBLE) from different origins.

SBLEs were prepared using 65% ethanol for hot reflux extraction of sea buckthorn leaves from different origins. The differences in their fingerprint profiles were analyzed by liquid chromatography. The colorimetric method was used to detect the inhibitory activity of Escherichia coli and Salmonella. The CCK-8 assay was employed to evaluate the activity of Vero cells infected with HSV-1/2. The luciferase reporter assay was conducted to assess the activity of NF-κB in TNF-α-induced NF-κB reporter (Luc)-HEK 293 cells.

SBLE from Datong and Huzhu exhibited better inhibitory effects on Escherichia coli (with EC₅₀ values of 184.3 and 211.6 μg·mL^-1, respectively) and Salmonella (with EC₅₀ values of 284.6 and 300.9 μg·mL^-1, respectively). SBLE from Datong, Pingan, Huzhu, Youyu, Heshun, and Zhangbei showed similar inhibitory effects on HSV-1 and HSV-2, with EC₅₀ values ranging from 27 to 36 μg·mL^-1. SBLE from Heshun, Zhangbei, and Huzhu exhibited better inhibition of NF-κB, with EC₅₀ values of 122.6, 137.1, and 154.1 μg·mL^-1, respectively.

The activities of SBLE from different origins vary, with SBLE from Datong exhibiting the best anti-Escherichia coli/Salmonella activity, SBLE from Youyu showing the best anti-HSV-1/2 activity, and SBLE from Heshun having the best NF-κB inhibitory effect.

To establish a simple and economical high performance liquid chromatography (HPLC) method for determination of the concentrations of ceftazidime, meropenem, ceftriaxone and cefoperazone in human plasma.

The analysis was performed using an Agilent Extend C₁₈ (4.6 mm × 250 mm, 5 μm) chromatographic column, and protein components were removed using ultrafiltration tubes. A gradient elution system consisting of acetonitrile and 50 mmol·L^-1 sodium dihydrogen phosphate (pH 2.4) was employed as the mobile phase. The column temperature was maintained at 35 °C, with a flow rate of 1 mL·min^-1. Detection was achieved using a dual-wavelength ultraviolet spectrophotometry at 250 and 300 nm.

The four β-lactam antibiotics exhibited good linearity within their respective ranges (r²> 0.999), with an average extraction recovery ranging from 68.98% to 83.50%. The intra-day precision RSD was between 0.06% and 2.67%, while the inter-day precision RSD was between 0.04% and 3.32%.

This study successfully established an HPLC method for the determination of the concentrations of ceftazidime, meropenem, ceftriaxone, and cefoperazone, which is suitable for monitoring the plasma concentrations of these four β-lactam antibiotics.

To establish a size exclusion high-performance liquid chromatography (SEC-HPLC) method for the determination of the purity of recombinant human basic fibroblast growth factor (rh-bFGF).

The purity of rh-bFGF solution was determined using TSK G2000SWXL column and high-performance liquid chromatography, and the specificity, repeatability, precision, detection limit, stability, and durability of the method were evaluated.

The blank solvent has no interference with the rh-bFGF main peak, and the degradation impurities under various destruction conditions didn't interfere with the determination of the rh-bFGF peak, and the purity index of the rh-bFGF peak was greater than 990. The repeatability and intermediate precision experimental results showed that the separation degree of impurities and the rh-bFGF main peak was greater than 1.5, and the RSD was 0.36% and 0.64%, respectively. The detection limit was 0.185 μg·mL^-1. The durability experiment results show that fluctuations in flow rate, column temperature, and wavelength had no effect on the detection results, and the RSD of each condition was less than 2.0%.

The SEC-HPLC method developed has the advantages of simple operation, accurate results, strong specificity, high stability and good applicability, and can be used for rh-bFGF purity determination.