To explore the efficacy and influencing factors of intraorbital electroacupuncture in the treatment of diabetic oculomotor nerve palsy.

The clinical data of patients with diabetic oculomotor nerve palsy who received intraorbital electroacupuncture therapy in the First Affiliated Hospital of Harbin Medical University from January 2020 to November 2024 were retrospectively analyzed. Demographic characteristics, ocular motor nerve palsy score, glycosylated hemoglobin, course of oculomotor nerve palsy, course of diabetes, number of treatments, and maximum deflection angle of diplopia in horizontal and vertical directions were extracted from the electronic medical record system. The T test/Mann-Whitney U test was used to compare the difference between the cured group and the non-cured group, and the binary logistic regression was used to analyze the influencing factors of the curative effect.

Of the 167 patients, 67 were cured, 96 were effective, and 4 were ineffective. The overall cure rate was 40.1%, and the total effective rate was 97.6%. The symptoms of diplopia were alleviated after treatment. There was a statistically significant difference in the median value of the maximum horizontal (α) and maximum vertical (β) diplopia angle before and after treatment (P <0.001). After treatment, the maximum value of α in 67 cured patients was 6°, and the minimum value was 0°, with a confidence interval of 1.289-2.598. The maximum value of β is 5°, the minimum value is 0°, and the confidence interval is 1.113 ~ 2.462. Univariate analysis showed that there were significant differences in ocular motor nerve palsy score, glycosylated hemoglobin, treatment times and treatment days between the cured group and the non-cured group (P < 0.05). Regression analysis showed that the number of treatments was an effective protective factor for acupuncture (β =0.050, P < 0.001). Glycosylated hemoglobin was a risk factor (β =-0.248, P = 0.026).

Intraorbital electroacupuncture can effectively improve the symptoms of ptosis, diplopia and eye movement disorder in patients with diabetic oculomotor nerve palsy. The curative effect is positively correlated with the mild degree of oculomotor nerve palsy, stable blood glucose control and sufficient course of treatment.

To investigate the mechanism of rhubarb by activating the PINK1/Parkin pathway to regulate mitophagy and intervene in inflammasome activation to inhibit systemic inflammatory response and protect intestinal structure in sepsis mice.

A total of 40 6-8-week-old C57BL/6J male mice were randomly divided into high-dose rhubarb group, medium-dose rhubarb group, low-dose rhubarb group, sepsis model group and blank group. The high-dose rhubarb group, the medium-dose group, the low-dose group, and the sepsis model group were all modeled by cecal ligation and perforation to establish a mouse model of sepsis, After successful modeling, each dose group of rhubarb was given 0.52 mL/day of rhubarb liquid by gavage every day for 7 days, and on the 8th day, the eyeball blood of mice in each group was collected after successful modeling, serum was prepared, and intestinal tissue was retained; HE staining was used to observe the histopathological changes of intestinal tissue, and the levels of TNF-α, IL-1β and IL-6 in serum were detected by ELISA, Western blot was used to detect the expression levels of NLRP3, PINK1, Parkin, LC3I, LC3II in ileal tissues of mice in each group.

HE staining showed that the intestinal mucosal epithelial cell injury was the most serious in the sepsis model group, with multiple ulcers formed, obvious intestinal villi with a large amount of necrosis and large sloughing, accompanied by mucosal bleeding and edema, inflammatory cell infiltration reached the muscularis mucosa, goblet cells were significantly reduced, paneth cells were significantly increased, and inflammatory cells infiltrated into the muscularis mucosa; Compared with the model group, the intestinal mucosal epithelial cell damage, microvilli destruction and inflammatory cell infiltration in the rhubarb group were improved, and the number of goblet cells increased, while the number of paneth cells decreased, and the inflammatory cell infiltration was basically limited to the lamina propria. The serum TNF-α, IL-1β, IL-6 level in the sepsis model group was significantly increased(P < 0.01), and the serum TNF-α, IL-1β, IL-6 level in the rhubarb group decreased after treatment(P < 0.05, P < 0.01). The expression levels of NLRP3, PINK1, Parkin, LC3I, LC3II were significantly increased in the intestinal tissues of mice in the sepsis model group(P < 0.01). After treatment, the level of NLRP3 in each dose group of rhubarb decreased(P < 0.01), which was negatively correlated with the dose, and the expression level of PINK1, Parkin, LC3I, LC3II increased to varying degrees(P < 0.05, P <0.01), which was positively correlated with the dose.

By inhibiting the activation of intestinal NLRP3 inflammasomes, rhubarb activates the PINK1/Parkin pathway and increases the degree of mitophagy, thereby reducing the production of systemic inflammatory factors and protecting the intestinal structure.

To study the inhibitory effect of Fuzheng Xiao'ai Formula I on colorectal cancer tumors in nude mice from the perspective of intestinal flora.

The CRC nude mouse model was constructed and 20 successfully modeled nude mice were randomLy divided into the model control group and low dose (17.47 g/kg), medium dose (34.94 g/kg), and high dose (69.89 g/kg) groups of Fuzheng Xiao'ai Formula I, with 5 mice in each group. The model control group was gavaged with normal saline, and each dose of Fuzheng Xiao'ai Formula I was gavaged with the corresponding concentration of decoction. After 2 consecutive weeks of intervention, the nude mice were sacrificed, the tumor bodies were completely dissected, and some tumor tissues were taken for HE staining. Based on the tumor weight, volume and pathological results, the medium dose group with better efficacy was screened out. Meanwhile, the cecal contents of this group and the model control group were collected for metagenomic sequencing analysis.

The tumor weight and volume of nude mice in each dose group of Fuzheng Xiao'ai Formula I decreased. HE staining showed that the density of tumor cells in the low, medium, and high dose groups of Fuzheng Xiao'ai Formula I decreased, accompanied by different degrees of necrosis. Metagenomic sequencing showed that compared with the model control group, the abundances of Parabacteroides, Bifidobacterium, Faecalibacterium, Clostridium auriscati, and Faecalibaculum rodentium in the medium dose group significantly increased (P < 0.05), and the abundances of Proteus, Citrobacter, Morganella, Enterobacter, Morganella morganii, and Enterobacter hormaechei significantly decreased (P < 0.05). Kyoto Encyclopedia of Genes and Genomes analysis found that the starch and sucrose metabolism pathway was significantly different between the two groups (P < 0.05), among which α-glucosidase, UTP-glucose-1-phosphate uridylyltransferase, phosphoglucomutase, and maltose α-D-glucosyltransferase were significantly downregulated in this pathway (P <0. 05).

Fuzheng Xiao'ai Formula I repairs the intestinal barrier and maintains the homeostasis of the intestinal environment by increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria. Down-regulating α-glucosidase in the starch and sucrose metabolism pathway may be a potential way for it to exert anti-colorectal cancer effects.

To observe the protective effect of Bushen Zhuangjin Decoction on cartilage damage in knee osteoarthritis (KOA) rats with kidney deficiency and blood stasis type, and to explore its molecular mechanism.

Seventy female SD rats were divided into 7 groups by a random digital table, with 10 in each. Except the normal group, the others were established kidney deficiency and blood stasis type KOA models. Each 1 rat was selected from the model group and the normal group, which were euthanized to verify successful modeling. Among the others, the positive drug group was given a dose of 5.4 mg/kg of etoposide by gavage. The low, medium, and high dose groups of Bushen Zhuangjin Decoction were given Bushen Zhuangjin Decoction 2.1, 4.2, and 8.4 g/kg by gavage respectively. SB203580 group was orally administered with SB203580 3.0 mg/kg. The model group was given 1 mL/100 g of physiological saline by gavage. The other 10 female SD rats were given an equal volume of physiological saline by gavage (normal group). All lasted for 30 days. Enzyme linked immunosorbent assay was used for detecting tumor necrosis factor-α (TNF)-α, interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels in joint fluid. Hematoxylin eosin (HE) staining was used to observe the pathological changes of cartilage tissues, and the Mankin scores were compared. Real time reverse transcription polymerase chain reaction (RT-qPCR) was used to detect p38 mitogen activated protein kinase (p38MAPK) and nuclear transcription factors in various groups of cartilage tissues-κB p65 (NF-κB p65), nucleotide binding oligomerization domain like receptor 3 (NLRP3), and aspartate proteolytic enzyme 3 (Caspase3) gene expressions. Western blotting (WB) was used for detecting p38MAPK and NF-κB p65, NLRP3, Caspase3 protein expressions and p-p38MAPK levels in cartilage tissues.

KOA models of kidney deficiency and blood stasis type were successfully established. The TNF-α, IL-1β, IL-6 levels of joint fluid, Mankin score, p38MAPK gene, NF-κB p65, NLRP3, Caspase3 expressions and p-p38MAPK in knee cartilage tissues in the model group were all higher than those in the normal group (P <0.01), of which the treatment groups were all lower than the model group (P <0.01), and except for Caspase3 expressions the indexes in the high-dose group of Bushen Zhuangjin Decoction were lower than the SB203580 group (P <0.01), and the effect of Bushen Zhuangjin Decoction was dose-dependent. The cartilage tissue of the model group rats showed severe pathological changes, and the treatment groups all showed relief, and the effects of the low-dose and positive drug groups of Bushen Zhuangjin Decoction, the medium dose group of Bushen Zhuangjin Dectoction and SB203580 group were basically equivalent.

Bushen Zhuangjin Decoction can alleviate the inflammatory response of cartilage tissue in KOA rats with kidney deficiency and blood stasis type, and it is speculated to inhibit p38MAPK/NF-κB pathway achieves this effect.

To observe the clinical efficacy of Huanglian Wendan Decoction in the treatment of pediatric multiple tic disorder (phlegm-heat-driven wind evidence).

The selected research subjects were 112 children with TS who were diagnosed and treated in Nantong Traditional Chinese Medicine Hospital from January 2024 to October 2024, and were randomly divided into an observation group and a control group with 56 cases each. During the treatment period, the children were instructed to have a regular schedule, eat properly, maintain a good emotional state, and avoid adverse stimuli. All children were given ear acupoint pressure bean therapy. On this basis, the control group was given oral tiapride tablets, while the observation group was given Huanglian Wendan Decoction in water. All children were treated continuously for 8 weeks. The condition improvement, TCM syndrome scores and serum dopamine (DA), Gamma-aminobutyric acid (GABA), norepinephrine (NE) levels before treatment, 2 weeks of treatment and 8 weeks of treatment were recorded and compared between the two groups. Compare the clinical efficacy of the two groups of children after 8 weeks of treatment.

Before treatment, there was no difference in YGTSS scores, TCM evidence points and serum DA, GABA, NE levels between the two groups of children (P >0.05). after 2 weeks and 8 weeks of treatment, the YGTSS scores, TCM syndrome integral and serum DA level of the two groups decreased, while the GABA and NE levels increased. And at each time point, the observation group was better than the control group. (P <0.05). After 8 weeks of treatment, the total effective rate was 92.86% (52/56) higher in the observation group than 73.21% (41/56) in the control group (P < 0.05).

Huanglian Wendan Decoction can effectively improve the tic symptoms of children with phlegm-heat and wind-driven TS, alleviate the children's TCM symptoms, and reduce the serum DA level, with remarkable clinical efficacy.