Objective: To investigate the mechanism of Yangxue Antai serum in regulating the release of exosomes from JEG - 3 cells to affect endothelial cells and endometrial cells.Methods: The exosome release inhibitor GW4869 was used to inhibit the release of exosomes from JEG - 3 cells, and the Yangxue Antai serum interfered with JEG - 3 cells, A co - culture system was established in which JEG - 3 cells or exosomes secreted by JEG - 3 cells were co - cultured with HEC-1 - A cells or HUVECs. The expression of VEGFA and p - VEGFR2 in HUVECs, the expression of EMT - related proteins E - cadherin, Vimentin, N - cadherin and Fibronectin as well as the expression of deciduation - related proteins BMP2, HOXA10, PRL and IGFBP1 in HEC -1 - A cells were measured by Western blot. The migration rate of HUVECs was measured by cell scratch assay. The invasion and migration ability of HUVECs were measured by Transwell assay, F - actin expression was measured by cytoskeleton assay, and the lumen length in HUVECs was measured by tubule formation assay.Results: Compared with Control group, the expressions of VEGFA and p - VEGFR2 in GW4869 group were significantly decreased. The expression of E - cadherin was significantly increased, while the expression of Vimentin, N - cadherin and Fibronectin were significantly decreased. The expressions of BMP2, HOXA10, PRL and IGFBP1 were significantly decreased. Cell invasion and migration ability were significantly decreased, the lumen length was decreased, and the expression of F - actin was significantly decreased. Compared with GW4869 group, the expressions of VEGFA and p - VEGFR2 in GW4869 + Yangxue Antai and GW4869 + exosome group were significantly increased. The expression of E - cadherin was significantly decreased, while the expression of Vimentin, N - cadherin and Fibronectin was significantly increased. The expressions of BMP2, HOXA10, PRL and IGFBP1 were significantly increased. Cell invasion and migration ability were increased significantly, the lumen length was increased, and the expression of F - actin was increased significantly.Conclusion: Yangxue Antai serum can affect the angiogenesis of endothelial cells, EMT and decidualization of endometrial cells by regulating the release of exosomes from JEG - 3 cells.

Exosome  /  Angiogenesis  /  Epithelial - mesenchymal transformation  /  Decidualization