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This study aims to investigate the mechanism by which circular RNA hsa_circ_0058493 mediates arsenic exposure-induced apoptosis in lung cells.
A549 and BEAS-2B cells were exposed to different concentrations of sodium arsenite (NaAsO2), and the half-maximal inhibitory concentration (IC50) of NaAsO2 was determined by the CCK-8 assay. The effects of three arsenic species (NaAsO2, MMA, DMA) on hsa_circ_0058493 expression were analyzed by real-time quantitative PCR (RT-qPCR). After silencing hsa_circ_0058493 with siRNA, cell viability, mitochondrial membrane potential, and apoptosis were evaluated using CCK-8, JC-1 staining, and Hoechst 33342/PI double staining, respectively. The JAK2/STAT3 signaling pathway and related protein expression were assessed by Western blotting.
NaAsO2 significantly reduced cell viability(IC50: 35.03 μmol/L for A549, 7.809 μmol/L for BEAS-2B) and specifically induced the upregulation of hsa_circ_0058493 (P<0.05), while MMA and DMA showed no significant effects. Downregulating hsa_circ_0058493 reduced cell viability, depolarized mitochondrial membrane potential, and increased apoptosis (P<0.05). Western blotting showed that depleting hsa_circ_0058493 suppressed JAK2/STAT3 phosphorylation and downregulated c-Myc, CyclinD1, XIAP, and BCL-2 expression levels (P<0.05).After downregulating hsa_circ_0058493, cells were treated with NaAsO2, resulting in a further decrease in cell viability, and STAT3 protein expression differed between the two cell lines.
Downregulation of hsa_circ_0058493 induced decreased cell viability and apoptosis, which were associated with inhibition of JAK2/STAT3 pathway activity, suggesting that hsa_circ_0058493 may be involved in arsenic-induced lung cell apoptosis by regulating the JAK2/STAT3 signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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