Full
Outline
To establish a method for the simultaneous determination of glyphosate and its metabolite aminomethylphosphonic acid in human urine by ion chromatography-tandem mass spectrometry(IC-MS/MS).
The urine sample was purified using an HLB solid-phase extraction cartridge. After diluted and filtered, the filtrate was used for analysis. Gradient elution was performed using a potassium hydroxide solution as the mobile phase, with separation on an Ionpac AS20 anion exchange column(250 mm×2 mm). Detection was carried out using electrospray ionization in negative ion mode with multiple reaction monitoring, and quantification was achieved via the external standard method.
Glyphosate and aminomethylphosphonic acid showed good linear relationships within the ranges of 0.5-50 μg/L and 4-100 μg/L, respectively, with correlation coefficients greater than 0.999. The method detection limits were 0.8 μg/L and 3.6 μg/L, respectively. The recovery rates of the method were 83.0% to 115.2%. The intra-day and inter-day relative standard deviations(RSDs) ranged from 1.2% to 7.8% and 3.5% to 9.2%, respectively.
This method is reproducible and accurate, and it is suitable for biological monitoring of glyphosate and its metabolite aminomethylphosphonic acid in urine samples of occupational populations.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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