Full
Outline
To understand the detection rate and molecular typing characteristics of human rhinovirus in influenza-like cases among children in Guizhou Province from 2023 to 2024.
Throat swab samples of 4 771 children influenza-like cases in Guizhou Province from 2023 to 2024 were collected. A total of 11 common respiratory pathogens, including rhinovirus (HRV), enterovirus (EV), adenovirus (ADV), Boka virus (HBoV), influenza virus (IFV), parainfluenza virus (HPIV), common coronavirus (HCoV), novel coronavirus (SARS CoV-2), metapneumovirus (HMPV), respiratory syncytial virus (RSV) and mycoplasma pneumoniae (MP), were detected by multiple fluorescent quantitative PCR. Epidemiological data were analyzed by Excel 2021 and SPSS 22 statistical software for HRV; Select qualified HRV positive samples for VP4/VP2 gene sequencing, use MEGA11 for gene typing, and construct a phylogenetic tree using adjacency method.
A total of 371 HRV-positive samples were detected, with a detection rate of 7.78%. No statistically significant difference in HRV detection rates was observed between different gender groups (P=0.669) or age groups (P=0.328). However, a statistically significant difference was found across seasons (P<0.001). The detection rate of HRV co-infection was 3% (143/4 771), with HRV and EV co infection detected the most, accounting for 27.97% (40/143), followed by HRV and HPIV co infection, accounting for 14.69% (21/143), and RSV co-infection, accounting for 12.59% (18/143). A total of 20 HRV VP4/VP2 gene sequences were obtained, including 16 HRV-A strains, 1 HRV-B strain, and 3 HRV-C.
HRV is an important pathogen causing influenza-like cases in children in Guizhou Province from 2023 to 2024. HRV-A is the main popular plant type. There is co-infection with HRV in the case, with co infection of two pathogens being the most common. The main co-infection viruses are EV, HPIV, and RSV.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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