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Outline
To investigate the therapeutic effect of Hanfangji formula on silica particle-induced pulmonary fibrosis in mice and to explore its potential mechanisms.
A mouse model of silicosis was established by a single intratracheal instillation of silica suspension. Seventy C57 mice were randomly divided into a blank control group, exposure group, low-,medium-, and high-dose Hanfangji groups, drug safety group, and positive control group (nintedanib). Mice were administered drugs by gavage for 56 days after exposure. Body weight, lung weight coefficient, and peripheral blood leukocyte counts were measured. Flow cytometry was used to determine the proportion of CD86+ macrophages in lung and spleen tissues. Western blot was performed to detect the expression of α-SMA and GSDMD-NT proteins in lung tissues. Histopathological changes and collagen deposition were assessed by HE staining and Masson staining.
Following silica exposure, the Ashcroft score of lung tissue in mice increased from 0.48±0.20 points in the control group to 6.72±0.28 points (P<0.01). The proportion of CD86+macrophages increased from 17.0%±1.6% to 40.2%±5.0% (P<0.001), accompanied by significant upregulation of α-SMA and GSDMD-NT protein expression. After intervention with Hanfangji Formula, all of the above indicators were markedly improved.Among them, the Ashcroft score in the medium-dose group decreased to 1.68±0.24 points(P<0.01).
Hanfangji formula can alleviate silica-induced pulmonary fibrosis in mice, which may be associated with inhibition of inflammatory responses, regulation of macrophage polarization, and reduction of GSDMD-mediated pyroptosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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