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Outline
To investigate the ameliorative effect and underlying molecular mechanism of Periplaneta americana extract (PAK) on silica-induced lung tissue injury in mice, so as to provide ideas and experimental evidence for the prevention and treatment of silicosis.
A silica-induced mouse model of silicosis was established. Using in vivo animal experiments combined with pathological staining, immunohistochemistry, toxicological evaluation of major organs, transcriptome sequencing, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and 16S ribosomal DNA high-throughput sequencing, we systematically evaluated the efficacy and underlying mechanisms of Periplaneta americana extract in ameliorating silica-induced pulmonary lesions.
At the experimental dose of 400 mg/kg/d, PAK alleviated inflammatory injury and collagen deposition in lung tissue of mouse models, and downregulated the expression of fibrosis-related proteins, with no obvious organ toxicity observed at this dose. Transcriptomic analysis revealed that PAK targeted 149 silica-induced toxicological genes, which were mainly enriched in inflammatory pathways such as the IL-17 pathway. Further qRTPCR assays confirmed that PAK inhibited the Il17a/f mediated inflammatory pathway and downregulated the expression of Tnf-α, Il6 and other proinflammatory factors. Meanwhile, PAK increased the relative abundance of beneficial bacteria including Akkermansia, and ameliorated intestinal flora dysbiosis in silicosis mice, which may be associated with the attenuation of lung tissue damage.
PAK can inhibit the IL17 signaling pathway and improve intestinal flora, thereby alleviating silicainduced pulmonary inflammation and injury, which provides a reference for further research on PAK and the clinical diagnosis and treatment of pneumoconiosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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