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To investigate the preventive effect and potential mechanism of intervention with Akkermansia muciniphila (AKK) isolates on dextran sulfate sodium (DSS)-induced acute colitis in mice.
Forty 6-8-week-old male C57BL/6J mice were randomly divided into a blank control group, a model group, an HX003 group, and an HX004 group. After 4 weeks of prophylactic intervention, acute colitis was induced by dextran sulfate sodium (DSS). The pathological changes of colon and spleen tissues in each group were observed, the serum LPS level was determined, and the mRNA transcription levels and protein expression levels of inflammatory factors IL-6, IL-10, IL-1β, and IFN-γ in mouse colon tissues were detected. The colonic contents of mice were collected, and 16S rRNA sequencing was used to analyze the structure and abundance changes of the intestinal flora.
Prophylactic intervention with AKK isolates alleviated colonic inflammation in mice to a certain extent. Serum lipopolysaccharide (LPS) levels in mice exhibited a decreasing trend (HX003, P=0.228). Both transcription and expression of IL-6 in colonic tissue showed downward trends. The transcription level of the pro- inflammatory cytokine IL-1β was significantly decreased (HX003, P<0.05), accompanied by a downward trend in its protein expression. In contrast, the transcription level of the anti-inflammatory cytokine IL-10 was markedly upregulated (HX003, P<0.001), with an increasing trend observed in its expression. Compared with the model group, the intervention groups displayed an upward trend in gut microbial diversity and a downward trend in the Firmicutes/Bacteroidetes ratio.
AKK isolates alleviate colonic inflammation in mice with acute colitis to a certain extent and improve the diversity and structure of their intestinal flora.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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