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To establish a QuEChERS-UHPLC-MS/MS method for the determination of residues of 45 antibiotics in eggs.
Samples were extracted using a 90% acetonitrile aqueous solution (containing 0.1% formic acid), and the supernatant was transferred to a QuEChERS tube (150 mg HLB-P + 50 mg PSA) for purification. The purified extract was concentrated to near dryness under nitrogen, and then reconstituted with 10% methanol aqueous solution (containing 0.1% formic acid). Target compounds were separated using a BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient elution of 0.1% formic acid in water and 0.1% formic acid in methanol as the mobile phase. Detection was performed in positive ion multiple reaction monitoring mode, and quantification was achieved using matrix-matched calibration curves.
Over the concentration range of 1.0 to 200 μg/L, all 45 target analytes exhibited good linear relationships in their regression equations. The method’s limit of detection was 1.0 μg/kg, with spiked recoveries ranging from 69.2% to 109.6% and relative standard deviations (RSD) between 1.2% and 15.8%.
This method is simple, rapid, and exhibits high accuracy and precision, making it suitable for the simultaneous detection of 45 antibiotics in eggs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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