To establish a training system for laboratory operators in Biosafety Level 2 laboratories, aimed at enhancing the knowledge level of personnel, standardizing operational skills, and reducing laboratory accidents.
The Delphi method was employed to conduct two rounds of consultations with 17 experts in the field of biosafety from Beijing, Shanghai,Xinjiang, Fujian, Anhui, Chongqing, Guangzhou, and Shenzhen to determine the indicator system, while the Analytic Hierarchy Process (AHP) was used to establish the weights of the indicators.
The response rates for the two rounds of questionnaires were 94.44% and 100.00%, respectively, with expert authority coefficients of 0.962 and 0.944. The Kendall's coefficient of concordance (W) was high (all P < 0.01). Ultimately, the training system was defined to include 5 primary indicators, 21 secondary indicators, and 83 tertiary indicators.
This training system is structurally complete and content-rich, demonstrating strong scientific validity and reliability, and can serve as a reference for the training and assessment of laboratory operators in Biosafety Level 2 laboratories.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |