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To study the effect of short-term antibiotics cocktail exposure on the intestinal flora induced by azomethane (AOM) in mice.
Forty male ICR mice aged 3 to 4 weeks were randomly divided into control group (Control group), AOM group (AOM group), antibiotic group (Abx group), and antibiotic + AOM group (AbxAOM group). During the gavage period, AbxAOM group and Abx group were administered with antibiotic solution, meanwile Control group and AOM group were administrated with corresponding volume of pure water twice a day for 2 weeks. During the period of intraperitoneal injection,AOM group and AbxAOM group were intraperitoneally injected with AOM solution, the Abx group and Control group were intraperitoneally injected with sterile 0.9% NaCl solution once a week for 4 weeks. The feces of mice were collected 5 weeks after intraperitoneal injection, and the intestinal flora of feces was analyzed by 16SrRNA sequencing.
The Chao1 index and ACE index in AbxAOM group were higher than those in AOM group. Compared with AOM group, the relative abundance of Firmicutes in AbxAOM group was significantly higher (P<0.05), while that of Bacteroidetes was significantly lower at the phylum level (P<0.05). Compared with AOM group, the relative abundance of Bacteroides and Prevotella in AbxAOM group decreased significantly (P<0.05), and the relative abundance of Alistipes, Clostridium XIVa and Blautia increased significantly at the genus level (P<0.05).
The changes of intestinal flora after antibiotic treatment suggest that specific microbial groups rather than single strains may play an important role in the occurrence and development of CRC induced by AOM in mice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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