PDF
Full
Outline
To establish a rapid and accurate method for the detection of Vibrio cholerae O1 and O139 toxigenic strains.
On the basis of multienzyme isothermal rapid amplification technology, primers and probes were designed according to the hemolysin coding gene, cholera toxin coding gene and O antigen coding genes of Vibrio cholerae O1 and O139, and the detection method was preliminary established. With DNA of different Serotypes of Vibrio cholerae and other bacteria used as templates, the specificity and sensitivity of the detection method was verified.
When the probe addition amount of the reaction system was 0.6μL as recommended by kit instructions, it could successfully amplify four target genes including hemolysin coding gene, cholera toxin coding gene, and O antigen coding genes of Vibrio cholerae O1 and O139. When the probe addition amount was respectively optimized to 1.0 μL, 1.0 μL, 1.0 μL, 0.8 μL of the above-mentioned four genes, the amplification efficiency was higher. After optimizing probe addition, the specificity of the detection method was verified and each primer probe combination did not cross react with non target bacterial DNA. When conducting sensitivity analysis of the detection method, the genomic detection sensitivity of different target genes was 70fg or 290fg. The above results showed that the sensitivity and specificity of the detection method meet the design requirements.
A new method for the detection of Vibrio cholerae O1 and O139 toxigenic strains has been established. This method has characteristics of strong specificity, high sensitivity, and short detection time. It can be applied to the routine detection and rapid detection of Vibrio cholerae, and has a positive role in the prevention of cholera.
PDF
46
18
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
