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To realize rapid qualitative and quantitative detection of 13 triterpenoids in Wolfiporia cocos from Yunnan by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry.
Triterpenoids in Wolfiporia cocos were extracted by ultrasonic extraction (methanol). The separation was carried on an ACQUITY UPLC © HSS C18 column (2.1 mm×50mm, 1.7μm) with, 10 mmol/L ammonium formate-0.1% formic acid aqueous solution and acetonitrile as mobile phase. The gradient elution flow rate was 0.5ml/min, with the column temperature of 30℃. Negative ion multiple reaction monitoring mode was used for detection. Qualitative and quantitative analysis using secondary mass spectrometry and accurate molecular weight.
13 triterpenoids in Wolfiporia cocos were good with in the linear ranges (r≥0.99). The spiked recoveries were from 80.35% to 116.92%, with relative standard deviations of 1.88% to 9.7%. 13 triterpenoids were detected in Wolfiporia cocos samples, and the total contents were between 79 433-85 900 mg/kg.
The method is simple, accurate, efficient and sensitive, and it is suitable for the qualitative and quantitative detection of 13 triterpenoids in Wolfiporia cocos.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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