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To explore the metabolic characteristics and potential biomarkers of vitamin D deficiency in children and adolescents, and to provide a basis for early identification and prevention of vitamin D deficiency.
Sixty pairs of children and adolescents with vitamin D deficiency and normal levels (matched by age and gender) from the nutrition and health monitoring project of rural school-age children in Guangzhou from March to May 2023 were selected as the research subjects. Based on the determination of serum 25(OH)D and serum untargeted metabolomics, differentially expressed metabolites (set threshold: FC value >1.25 or <0.8, VIP value >1) were screened, and metabolic pathways were analyzed. Logistic regression was applied to screen potential biomarkers, and ROC curves were drawn to evaluate their accuracy.
56 differentially expressed metabolites (38 up-regulated and 18 down-regulated) were identified. The analysis of metabolic pathways showed that the steroid hormone biosynthesis pathway was statistically significant(P=0.001, QFDR=0.0982). Logistic regression analysis results showed that 12 potential biomarkers such as 7,8-epoxy-4Z,10Z,13Z,16Z,19Z-(OR=1.95, 95% CI: 1.20-3.17) had statistical significance in the association with vitamin D deficiency, and the AUC in the training set was 0.922(95% CI: 0.865-0.978), and in the test set was 0.910 (95% CI: 0.809-1), with high accuracy.
This study revealed the metabolic pathways related to vitamin D deficiency in children and adolescents and identified 12 potential biomarkers, providing a basis for clarifying the metabolic characteristics and carrying out early identification of vitamin D deficiency.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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