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To establish a method for detecting four arsenic species—As (III), DMA, MMA, and as (V)—in seafood using liquid chromatography-atomic fluorescence spectrometry (LC-AFS).
The detection conditions were optimized, including the type and concentration of the carrier liquid, concentration of potassium borohydride, lamp current, negative high voltage, mobile phase concentration, and pH. Parameters of the established detection method were evaluated, including linear range, accuracy, precision, and detection limit.
Under the conditions of 5% hydrochloric acid, 5 g/L sodium hydroxide-20 g/L potassium borohydride, a negative high voltage of 270 V, lamp main current of 60 mA, mobile phase A as ammonium dihydrogen phosphate (1 mmol/L, pH 8.5), and mobile phase B as ammonium dihydrogen phosphate (25 mmol/L, pH 8.0), the linear range for As(III), DMA, MMA, and As(V) was 0-100 μg/L, with correlation coefficients exceeding 0.999. The relative standard deviation (RSD) for retention time ranged from 0.2% to 1.2%, while the RSD for peak area ranged from 2.1% to 4.4%. The recovery rate for spiked samples was between 81.2% and 111.6%.
The method demonstrates high sensitivity, accuracy, and precision, with a short analysis time and high detection efficiency, while requiring minimal reagent usage, yielding satisfactory results.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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