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To elucidate antibacterial effects and mechanism of Rosa sertata×Rose rugosa methanol extraction (RME) against extended-spectrum β-lactamases producing Klebsiella pneumoniae (ESBL-KP).
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of RME against various ESBL-KP strains were determined by micro broth dilution method. Impact of RME on the growth, cell membrane and extended-spectrum β-lactamases (ESBLs) activity of ESBL-KP was evaluated by growth and time-kill curves, scanning electron microscopy and nitrocefin hydrolysis test, seperately. Components of the extracts were analyzed using Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-Q-TOF MS).
The MIC of RME was 1 mg/ml for standard strains and 8-32 mg/ml for ESBL-producing strains. Concentrations of RME at 1/4 MIC, 1/2 MIC, and MIC could inhibit bacterial growth, with higher concentrations showing stronger effects. For D141 strain, treatment with RME at 4MIC concentration for 4 hours resulted in cell membrane collapse, bacterial agglutination, and a 77.49% inhibition rate of ESBLs activity; no bacterial growth was observed after 24 hours of this treatment. Totally, 1 550 compounds were identified in RME, with quinic acid, tricoumaroyl spermidine, thioetheramide-PC, and kaempferol-3-O-rutinoside being the predominant components.
RME can inhibit and kill ESBL-KP through disrupting its cell membrane and inhibiting the enzymatic activity of ESBLs, which provided a scientific basis for further research on Rosa sertata × Rosa rugosa.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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