PDF
Full
Outline
To investigate whether sub chronic exposure to Benz[a]pyrene (BaP) induces ferroptosis in mouse hepatocytes, leading to liver injury, thereby providing a basis for further study of the hepatic toxicity mechanisms of BaP.
Forty-eight male C57BL/6 mice aged three weeks were randomly divided into six groups: control group (corn oil),low-dose BaP group (2.5 mg/kg), medium-dose BaP group (5 mg/kg), high-dose BaP group (10 mg/kg), ferroptosis inhibitor Fer-1 group (3-amino-4-cyclohexylaminobenzoic acid ethyl ester, 1 mg/kg), and high-dose BaP + Fer-1 group (10 mg/kg + 1 mg/kg). BaP was administered via gastric gavage dissolved in corn oil, and Fer-1 was injected intraperitoneally every other day for 90 days. Hepatic structure and collagen fiber deposition were observed using HE and Masson staining, while transmission electron microscopy (TEM) was used to examine the ultrastructure of hepatocytes. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mouse liver tissues, as well as tissue iron, malondialdehyde (MDA), glutathione (GSH) content, and glutathione peroxidase (GSH-Px) activity, were measured using kits. Western blotting was employed to detect the levels of glutathione peroxidase 4 (GPX4) and acyl-CoA synthetase long-chain family member 4 (ACSL4) in mouse liver tissues.
Compared to the control group, the high-dose BaP group exhibited a decrease in body weight (t=4.921, P=0.006) and a reduction in liver coefficient in both medium and high-dose BaP groups (t medium=4.967, P<0.05; t high=6.568, P=0.001). The ALT and AST levels in liver tissues of BaP-treated mice were elevated (F ALT=218.200, P<0.001; F AST=421.200, P<0.001). HE staining revealed varying degrees of hepatocyte disarray, sinusoidal dilation, congestion, and inflammatory infiltration in the liver of BaP-treated mice, while Masson staining indicated collagen fiber deposition. Iron content in the liver tissues of BaP-treated mice increased (W=41.730, P<0.001), and both GSH concentration and GSH-Px activity decreased (W GSH=49.640, P<0.001; F GSH-Px=252.400, P<0.001), with an increase in MDA concentration (F=207.700, P<0.001). The expression level of GPX4 protein in the liver of BaP-treated mice decreased (F=56.790, P<0.001), while the expression level of ACSL4 protein increased (F=429.400, P<0.001). Compared to the high-dose BaP group, the high-dose BaP + Fer-1 group showed improvements in body weight changes (t=5.970, P<0.001), liver coefficient (t=11.510, P<0.001), morphological changes in the liver, and levels of ALT (q=21.730, P<0.001), AST (q=32.870, P<0.001), tissue iron (t=5.045, P=0.009), GSH (t=10.600,P<0.001), GSH-Px (q=9.977, P<0.001), MDA (q=21.580, P<0.001), as well as the expression levels of ACSL4 (q=8.629, P<0.001) and GPX4 (q=5.146, P=0.03) proteins.
Sub chronic exposure to BaP can induce ferroptosis in mouse hepatocytes, resulting in liver injury.
PDF
55
25
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
