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To study the effect and mechanism of nicotinamide mononucleotide (NMN) in alleviating silica-induced lung injury, and to provide a new option for the adjuvant treatment of silicosis.
The male C57BL/6 mice were randomly divided into control group, SiO2 group, and NMN intervention group according to body weight, with 10 mice in each group. Mice were given SiO2 (50 mg/ml, 80 μl) by tracheal instillation to construct a mouse model of silicosis, and NMN (1 000 mg/kg) was given by gavage for 7 days and 28 days. Histopathological staining was used to assess lung lesions, and transcriptomic sequencing was used to analyze gene expression and screen for key genes.
NMN intervention alleviated SiO2-induced lung injury. Gene clustering results showed that NMN intervention improved SiO2-induced gene expression disorders to a certain extent, and the improvement effect of 28 days was better than 7 days of NMN intervention. After 7 and 28 days of SiO2 exposure, 1 163 genes were up-regulated in the SiO2 group compared with the control group, and were down-regulated in the NMN group compared with the SiO2 group, of which 445 were differential genes. Similarly, 1 657 genes were down-regulated in the SiO2 group compared with the control group, and were up-regulated in the NMN group compared with the SiO2 group, of which 571 were differential genes. The main GO term of the above differential genes was RNA polymerase and transcriptional regulation, and main KEGG pathways were IL-17 signaling pathway (P<0.001), cytokine-cytokine receptor interaction (P<0.001), Th17 cell differentiation (P<0.001), TNF signaling pathway (P<0.001). According to the number of pathways involved in the regulation of differential genes, the TOP8 hub genes were Mapk11, Mapk12, Il1b, H2-Ob, Csf2, Shc2, Mmp9, Ccl25.
NMN alleviates lung injury in silicosis mice by regulating immunity.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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