PDF
Full
Outline
To investigate the variations in the species composition and functional attributes of intestinal microbiota in individuals suffering from metabolic fatty liver disease in Tibetan regions, and to provide a research basis for the prevention and treatment of metabolic fatty liver disease in Tibetan population.
Based on the follow-up survey data of the Southwest region natural population in Lhasa, a total of 103 subjects were included for follow-up analysis according to the guidelines for the prevention and treatment of metabolic-associated (non-alcoholic) fatty liver disease (2024 edition). The demographic information, anthropometric indicators, blood biochemical indicators, and fecal samples were collected, and DNA was extracted for metagenomic sequencing analysis. The composition of intestinal flora in patients with metabolic fatty liver disease and healthy people was compared by bioinformatics methods. The metabolic pathways and their coding genes were analyzed by genome encyclopedia (KEGG) . Linear discriminant analysis was used to screen the differential flora, and Spearman rank correlation and linear regression were used to explore its relationship with related phenotypes.
The alpha diversity of patients with metabolic fatty liver was significantly reduced (W=858, P<0.001). The abundance of Bacteroidetes in the intestinal tract of patients with metabolic fatty liver was significantly lower than that of normal people (H=8.990, P=0.003). These include Bacteroides uniformis, Bacteroides fragilis, Bacteroides ovatus, and the butyrate-producing Porphyromonas Phocaeicola salanitronis (LDA>3, P<0.050). Fusobacterium varium (LDA=2.16, P=0.024) was significantly enriched in MAFLD population and positively correlated with multiple disease phenotypes. Glucose and lipid metabolism-related pathways and KO genes were also significantly enriched in patients with metabolic fatty liver.
The intestinal microbiota and metabolic function of Tibetan patients with metabolic fatty liver disease are significantly changed, and the disruption of intestinal flora balance may also have a close association with the onset and progression of metabolic fatty liver disease among Tibetan patients.
PDF
57
22
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
