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To explore the clustering of drug-resistant Mycobacterium tuberculosis (MTB) in Zigong from 2018 to 2020, analyze the genetic relationship between strains, and speculate whether there is a potential correlation between drug-resistant strains isolated from different patients, so as to provide a reference for the prevention and treatment of drug-resistant tuberculosis in Zigong.
The drug-resistant MTB strains isolated from Zigong from 2018 to 2020 were used as the research objects and the standard MIRU-VNTR 12-loci method was used for typing study. The band sizes of the amplified products were analyzed by PCR and agarose gel electrophoresis to obtain the number of replicates of different loci of the strains. The data were imported into the MIRU-VNTRplus database and the SITVIT2 website to construct the phylogenetic tree diagrams and the minimum spanning trees, and the global source analysis of the isolated strains was performed. The discriminatory ability of the 12-locus method was evaluated by locus polymorphism analysis, and the local prevalence and transmission of drug-resistant TB were analyzed by calculating the cluster formation rate and recent transmission rate.
The overall resolution HGI index of MIRU-VNTR standard 12-loci typing was 0.974. MIRU26 and MIRU31 showed high polymorphism; MIRU10, MIRU16, MIRU39, and MIRU40 showed moderate polymorphism, and the remaining loci showed low polymorphism. The most concentrated locus was MIRU2, and the most dispersed locus was MIRU26. The 120 drug-resistant strains contained 77 genotypes, with a clustering rate of 46.67 % and a recent transmission rate of 34.17 %. The minimum spanning tree showed that 120 drug-resistant strains could be divided into three clonal complexes, CC1, CC2, and CC3, according to the VNTR typing results. Among the 120 valid strains, 12 genotypes of 32 strains could be found on the SITVIT2 website, and the corresponding 12 MIT numbers were distributed in 14 countries on five continents worldwide.
The standard VNTR-12 locus method resolves drug-resistant tuberculosis strains well. There is a certain range of transmission of drug-resistant TB in Zigong, and the recent transmission rate is high, targeted surveillance is helpful in the prevention and control of tuberculosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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