Full
Outline
To investigate the role and mechanism of FABP5 in pulmonary inflammation associated with silicosis by analyzing multi-omics data in conjunction with a silicosis mouse model.
Single-cell sequencing was performed to analyze the potential role of FABP5 in occupational respiratory diseases; a silicosis mouse model was used to validate the relationship between FABP5 and pulmonary inflammation in silicosis; intra-abdominal injection of FABP5 inhibitors to modulate FABP5 function; and employed hematoxylin-eosin staining, immunohistochemistry, Western blotting, real-time quantitative PCR, and transcriptomic sequencing to investigate the role and potential mechanisms of FABP5 in silica-induced lung tissue inflammation in mice.
Single-cell sequencing revealed that FABP5 expression was elevated by 3.10-fold (P<0.01) and 4.71-fold (P<0.05) in lung tissue from patients with pneumoconiosis and in a mouse model of silicosis, respectively, and was highly enriched in macrophages; Transcriptomic sequencing revealed that, compared to the silicosis model group, inhibition of FABP5 downregulated the expression of inflammatory genes such as TNF, IL-1α, and IL-1β, with the relative protein expression levels of TNF-α and IL-1β decreasing by 4.67-fold (P<0.001) and 0.25-fold (P<0.01), respectively.
FABP5 is a key pathogenic factor in silicosis-induced pulmonary inflammation. In vivo inhibition of FABP5 alleviates pulmonary tissue inflammation in silicosis, providing insights for research into the mechanisms and clinical management of silicosis.
202
88
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
