Full
Outline
To investigate the contamination status of Listeria monocytogenes in commercial food in Hainan province and characterize the antimicrobial resistance of the isolated strains. This study aims to provide baseline data for foodborne L. monocytogenes surveillance and scientific evidence for the prevention and control of listeriosis.
From 2015 to 2024, 8 878 commercial food samples were collected in Hainan Province and screened for L. monocytogenes. Thirty strains isolated from 2019 to 2024 underwent whole genome sequencing, and 26 were tested for antimicrobial susceptibility.
A total of 38 L. monocytogenes strains were detected in this study, and the positive rate of 0.43%. The highest annual rate occurred in 2022 (1.29%, 15/1 160), and the peak seasonal rate was in the second quarter (0.91%, 24/2 639). Geographically, Baisha County (1.48%, 2/135) showed the highest rate. Among food categories, meat and meat products exhibited the highest contamination (1.06%, 22/2 072). Retail processing stores had highest facility-level rate (1.67%, 2/120), while the circulation stage showed the highest rate among sampling links (0.49%, 33/6 783). Antimicrobial susceptibility testing identified one L. monocytogenes isolate with dual resistance to tetracycline and erythromycin. All other isolates were susceptible to all tested antibiotics. Lineage II predominate (66.67%, 20/30), with serogroup IIA as the most common type (50.0%, 15/30). ST8 was the predominant sequence type (20.0%, 6/30). All strains carried the virulence island LIPI-1, and 56.7%(17/30) strains carried the stress survival island SSI-1.
L. monocytogenes is present in meat, meat products and other foods in Hainan Province at a low overall contamination. ST8 is the predominant sequence type, and the isolates remains largely susceptible to antibiotics. These findings highlight the need to enhance monitoring of food contamination, particularly in meat and meat products, to detect potential hazards early and track evolving antimicrobial resistance pattern for effective listeriosis prevention and control.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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