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Outline
The infectivity of plasma from blood donors with occult hepatitis B virus infection (OBI) in human hepatocyte cells was investigated through an in vitro hepatitis B virus (HBV) infection model using the L-02 hepatocyte cell line.
Among 54 446 blood donors, 28 OBI plasma samples were collected as the experimental group, and 28 plasma samples from hepatitis B surface antigen (HBsAg)-positive blood donors were randomly selected as the control group.The control group samples were used to infect L-02 hepatocytes to establish an in vitro hepatocyte infection model. The infectivity of the experimental group samples to L-02 hepatocytes was studied using the same experimental method.
Except for one case, HBV DNA was detected in the lysate of L-02 hepatocytes in all 27 specimens of the control group after infection. In contrast, only one of the 28 OBI specimens had detectable HBV DNA in the lysate of hepatocytes.
The probability of OBI donor plasma infecting hepatocyte cells is much lower than that of HBsAg-positive donor plasma, but there is still a risk of HBV transmission through blood transfusion. The presence of high-titer hepatitis B surface antibodies may be one of the key factors in preventing HBV transmission through blood transfusion from OBI.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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