To analyze the impact of the separation of medicine and pharmacy and the integrated reform of medical expenses on medical service revenue in a district of Beijing.

Utilizing monitoring data from January 2016 to April 2024, a time breakpoint regression model was constructed for analysis.

Following the implementation of the separation of medicine and pharmacy reform, the proportion of medical service revenue and the average medical service revenue per visit in secondary and tertiary hospitals significantly increased (P<0.05). After the implementation of the integrated reform of medical expenses, the average medical service revenue per visit and the proportion of service revenue in secondary hospitals also saw significant increases (P<0.05).

After reform, the total amount and proportion of medical service revenue have significantly increased, with further optimization of the revenue structure and a more adequate reflection of the value of technical services. However, the proportion of medical service revenue remains relatively low. It is recommended to enhance the reform linkage mechanism, strengthen the supervision of medical services, promote the balanced distribution of medical resources, and focus on improving the service capacity of primary healthcare institutions.