The current national standard employs the phenol-sulfuric acid method for quantifying the curdlan content. However, due to inadequate dispersion of some curdlan particles in the solvent caused by agglomeration during dissolution in a 0.1mol/L sodium hydroxide solution, the results of content detection were found to be unstable. The samples were treated by magnetic stirring, homogenizer dispersion and ethanol dispersion. It was found that: The ethanol effectively disperses the curdlan sample in the sodium hydroxide solution, preventing it from agglomerating. The ethanol method effectively and efficiently addresses the pain point in determining the content of curdlan, offering valuable insights for enhancing the detection methodology.

College pharmacy teaching laboratory is an important place to train pharmaceutical technology practical talents, involving many disciplines, equipment and chemicals, which will bring a large number of unsafe factors, and a little carelessness will cause personal, property and environmental safety accidents. Therefore, safety management should be strengthened. Based on the practice of laboratory safety management, this paper analyzes the current situation and problems of safety management, and believes that it can be improved from the aspects of safety system, safety education, hardware construction and targeted management of each laboratory, so as to effectively improve the safe operation of the laboratory.

Objective Comparing two detection methods for chlamydial trachomatis in the genital tract (GeneXpertⓇ CT/NG and Roche® Cobas 4800 CT/NG), we summarize their respective advantages and disadvantages to provide references for the promotion and application of these two methods. Methods During a field survey conducted in a sample hospital, a convenience sampling method was employed to select 60 patients. Vaginal swabs were collected from these patients for testing using the GeneXpert® CT/NG system, while urine samples were collected for testing with the Roche® Cobas 4800 CT/NG system. Staff members were tasked with recording and compiling data related to the testing process, including the time taken for testing, the cost of testing, and the positive detection rates. This data was then used to compare the two testing methods. Results The testing time for Roche® Cobas 4800 CT/NG is 293 minutes, while the testing time for GeneXpertⓇ CT/NG is 92.95 minutes. In other words, the testing time for Roche® Cobas 4800 CT/NG is 3.15 times longer than that of GeneXpert® CT/NG; When the number of tests conducted in the sample hospital reaches 3000 cases, the cost of Roche® Cobas 4800 CT/NG testing is approximately 1/5 of that of GeneXpert® CT/NG testing. As the number of tests increases, when the volume reaches 200,000 cases, the unit cost of Roche® Cobas 4800 CT/NG testing is approximately 1/3 of the unit cost of GeneXpert® CT/NG testing; Among the 60 participants in this study, 11 cases of CT positive patients were detected by GeneXpert® CT/NG, with a CT positive rate of 0.183. Roche®Cobas 4800 CT/NG detected 10 cases, with a CT positive rate of 0.167. Conclusion The GeneXpert® CT/NG test has a significant time advantage, as it takes less time and provides faster results; The Roche® Cobas 4800 CT/NG test has a cost advantage, with lower detection costs; The GeneXpert® CT/ NG test has a higher positive detection rate.

Recombinant collagen dressings are currently a popular skin care product in the market, which can form a protective layer on the wound and act as a physical barrier. Used for the care of non chronic wounds (such as superficial wounds, laser/photon/fruit acid skin replacement/micro plastic surgery wounds), providing a microenvironment for wound healing. The detection of recombinant collagen protein in application products is one of the reliable indicators to ensure product quality. This article investigates the applicability of the identification method for a recombinant collagen liquid dressing. The applicability of a identification method for a recombinant collagen liquid dressing was studied. Peptide map method, $\mathrm{N}$ -terminal amino acid sequence method, and isoelectric focusing electrophoresis method were used to identify the recombinant collagen in the experimental sample, and the detection results showed that the peptide map method and N-terminal amino acid sequence method were applicable to the sample. However, the isoelectric focusing electrophoresis method did not detect any bands, so this method is not applicable. When choosing the identification method for recombinant collagen, enterprises should consider the actual situation of the product and adopt appropriate methods.

Objective To establish a method for the determination of six phthalates [diisobutyl phthalate (DIBP), di-n-butyl phthalate (DBP), di(2-butoxy)ethyl phthalate (DBEP), dicyclohexyl phthalate (DCHP), di(2-ethyl)hexyl phthalate (DEHP) and diisononyl phthalate (DINP)] in semi-solid food by gas chromatography-mass spectrometry (GC-MS/MS). Methods The optimization of chromatography and mass spectrometry conditions, the comparison of pretreatment extraction methods, and the selection of purification columns were carried out. The samples were extracted with an organic solvent, and the supernatant was purified by a solid phase extraction column made of ethylenediamine-N-propylsilane/silica gel (PSA/Silica) composite packing glass, and separated by a capillary column TG-5MS (${{30}\mathrm{\;m}}\times {0.25}\mathrm{\;{mm}}\times {0.25}\mathrm{\;{\mu m}}$). The optimal conditions were selected by mass spectrometry full scan (SCAN) mode, and then by feature selective ion monitoring scan mode (SIM) detection, the retention time and qualitative ion fragment abundance ratio were qualitatively analyzed, and the external standard method was used for quantitative analysis. Results The separation was completed within 26.5 min. Linear range: DINP had a good linear relationship in the concentration range of${0.2}\sim {5.0}\mathrm{{\mu g}}/\mathrm{{mL}}$, with$\mathrm{r}> {0.9995}$, and the other five species had a good linear relationship in the range of ${0.02}\sim {0.50\mu}\mathrm{g}/\mathrm{{mL}}$, with$>\mathrm{r}{0.9995}$。The limit of quantification was${9.0}\mathrm{{mg}}/\mathrm{{kg}}$, DINP was ${0.3}\mathrm{{mg}}/\mathrm{{kg}}$, and the others were ${0.5}\mathrm{{mg}}/\mathrm{{kg}}$, and the three-level standard solution was added to the two dipping materials and bottom materials, respectively: DINP was 20,40 and ${60}\mathrm{{mg}}/\mathrm{{kg}}$, and the others were 4,6 and 8 $\mathrm{{mg}}/\mathrm{{kg}}$, and the recovery rates were 89.1%~101%. The RSD % was less than 3.8%. Conclusion This method has simple pretreatment, good selectivity, sensitivity and accuracy, and can be widely used for the determination of plasticizers in semisolid composite seasonings.

This study will start with the current problems in the safety and management of multi campus university laboratories, and explore new methods and approaches for intelligent management and security system design of laboratories based on the perspective of the Internet of Things. We will use Internet information technology to build a resource sharing, fully open and efficient experimental teaching platform, build a laboratory management system based on the big data era, monitor the whole process before, during and after the experiment through the collection and analysis of data generated by the use and operation of various instruments and equipment, find problems in the experiment in time, carry out targeted guidance, effectively warn laboratory hazards, improve laboratory safety management efficiency, push laboratory management to a higher level, and achieve the win-win effect of talent training, scientific innovation, and safety management.

With the rapid development of information technology, laboratory information management has become a key means to improve research efficiency and quality. This article delves into the application and significant benefits of laboratory information management in data processing, resource utilization, team collaboration, and research innovation. By implementing electronic data management, online resource scheduling, cross regional collaboration platforms, and advanced data analysis, information management not only improves research efficiency, but also promotes the improvement of research quality and innovation capabilities. The further development of information technology will make laboratory management more efficient, secure, and collaborative, providing strong support for scientific research.

Objective By means of High Performance Liquid Chromatography (HPLC), the extraction method and content determination method of $\alpha$ -cyperone were explored to achieve the purpose of accuracy, reliability, convenience and easy operation. Methods High performance liquid chromatography was used. The chromatography was performed on Agilent Eclipse Plus $\left({{150}\times {4.6}\mathrm{\;{mm}},5\mathrm{\;{\mu m}}}\right)$ silica gel column with mobile phase consisted of methanol-water (70:30) and UV-VIS spectrometer. The flow rate was ${1.0}\mathrm{{mL}}/\mathrm{{min}}$ . The detection wavelength was ${240}\mathrm{\;{nm}}$ . The column temperature was ${35}^{\circ }\mathrm{C}$ . Results The results showed a good linear relationship in the linear range of ${0.02314}- {0.46287}\mathrm{{mg}}/\mathrm{{mL}}$ with ${\mathrm{R}}^{2}= 1$ , the average recovery rate of ${99.84}\%$ and relative standard deviation (RSD)=0.09%. Conclusion The method has the advantages of simple operation, high sensitivity, good repeatability, accuracy and reliability, and can be used to determine the content of $\alpha$ -cyperone in order to control the quality of cyperone effectively.

Objective Comparison of two methods for determining ammonium ion content in ambient air${\mathrm{{PM}}}_{2.5}$. Methods $1/4$ of the quartz filter membrane used to collect ambient air particles${\mathrm{{PM}}}_{2.5}$ was cut into pieces, and ultrasound extracted by ultra-pure water, then centrifuged and filtered, and last the content of ammonium ion in${\mathrm{{PM}}}_{2.5}$ was respectively determined by flow injection method and ion chromatography. Result The fitting curve of${\mathrm{{NH}}}_{4}^{+ }$ measured by ion chromatography in the range of${0.5}\sim {10.0}\mathrm{{mg}}/\mathrm{L}$ is$\mathrm{y}= {0.0710}+ {0.2214}\mathrm{x}- {0.0058}{\mathrm{x}}^{2},\mathrm{r}= {0.9997}$ . The detection limit of${\mathrm{{NH}}}_{4}^{+ }$ in${\mathrm{{PM}}}_{2.5}$ is calculated to be${0.017}\mathrm{{\mu g}}/{\mathrm{m}}^{3}$ , with a quantification limit of${0.068\mu }\mathrm{g}/{\mathrm{m}}^{3}$ , the recovery rate is between${91.0}\%\sim {96.6}\%$ , and the RSD is${0.05}\%\sim {0.08}\%\left({\mathrm{n}= 6}\right)$ . The linear relationship of ammonium ion concentration measured by flow injection analysis in the range of${0.02}\sim {1.00}\mathrm{{mg}}/\mathrm{L}$ is good, with$\mathrm{y}= {22.576}\mathrm{x}+ {0.1333},\mathrm{r}= {0.9999}$ . The detection limit is${0.003}\mathrm{{\mu g}}/{\mathrm{m}}^{3}$ , and the quantification limit is${0.012\mu }\mathrm{g}/{\mathrm{m}}^{3}$ with the recovery rate of${105}\%\sim {114}\%$ and the RSD between${0.54}\%\sim {2.06}\%\left({\mathrm{n}= 6}\right)$ . Using the two methods to measure 16 filter membrane samples, there was no statistically significant difference in the measurement results$\left({\mathrm{t}= {1.080},\mathrm{P}> {0.05}}\right)$. Conclusion Both of the two methods have a good linear relationship, with good precision and accuracy. Compared with ion chromatography, flow injection analysis has the advantages of lower detection limit and shorter detection time.

Objective At present, the systematic study of heavy metals releasing law from ceramic disc sealed water faucet is lack in domestic. At the same time, nozzle production enterprises also lack relevant knowledge, so it is necessary to study this to provide a scientific basis for the green and sustainable development of the nozzle industry. Methods Through the laboratory simulation method, the inductively coupled plasma mass spectrometer was used to analyze and test the precipitation of heavy metal pollutants in the nozzle, and systematically studied the precipitation law of heavy metal pollutants in the ceramic sheet sealed nozzle. Results Through testing and analysis, the material of the surface nozzle material also determines the precipitation type of heavy metal pollutants. The immersion time and the concentration of inorganic salt in water quality are directly proportional to the precipitation of heavy metals. The lead brass nozzle effectively reduces the precipitation of lead and chromium heavy metals through the lead removal process; the precipitation of lead is reduced by 81% to 86%, and the precipitation of chromium is reduced by 52% to 56%. Conclusion Enterprises can choose suitable materials and processes to produce nozzles according to their own conditions. Consumers should avoid exposure to excessive heavy metals according to their use.