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Objective By means of High Performance Liquid Chromatography (HPLC), the extraction method and content determination method of $\alpha$ -cyperone were explored to achieve the purpose of accuracy, reliability, convenience and easy operation. Methods High performance liquid chromatography was used. The chromatography was performed on Agilent Eclipse Plus $\left({{150}\times {4.6}\mathrm{\;{mm}},5\mathrm{\;{\mu m}}}\right)$ silica gel column with mobile phase consisted of methanol-water (70:30) and UV-VIS spectrometer. The flow rate was ${1.0}\mathrm{{mL}}/\mathrm{{min}}$ . The detection wavelength was ${240}\mathrm{\;{nm}}$ . The column temperature was ${35}^{\circ }\mathrm{C}$ . Results The results showed a good linear relationship in the linear range of ${0.02314}- {0.46287}\mathrm{{mg}}/\mathrm{{mL}}$ with ${\mathrm{R}}^{2}= 1$ , the average recovery rate of ${99.84}\%$ and relative standard deviation (RSD)=0.09%. Conclusion The method has the advantages of simple operation, high sensitivity, good repeatability, accuracy and reliability, and can be used to determine the content of $\alpha$ -cyperone in order to control the quality of cyperone effectively.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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